Molecular mechanisms of resistance to some pesticides in the two-spotted spider mite Tetranychus urticae (Acari: Tetranychidae) from Egypt | ||||
| Catrina: The International Journal of Environmental Sciences | ||||
| Article 11, Volume 11, Issue 1, 2015, Page 75-80 PDF (431.19 K) | ||||
| Document Type: Original Article | ||||
 View on SCiNiTO | ||||
| Authors | ||||
Mohamed Salman1; Akram Abouzied  2; Ayat El-Gammal1; Ahmed Abo-Ghalia2
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| 1Plant Protection Research Institute, Agriculture Research Center, Egypt | ||||
| 2Department of Zoology, Faculty of Science, Suez Canal University, Ismailia 41522, Egypt | ||||
| Abstract | ||||
| The two-spotted spider mite, Tetranychus urticae (Acari: Tetranychidae), is the major pest affecting crops in Egypt. To date, synthetic acaricides are used extensively to control it. These include two specific acaricides: i.e. abamectin and chlorfenapyr compounds that are toxic to T. urticae and used widespread in Egypt. Nevertheless, progressive insensitivities to these acaricides are problematic in many areas under field conditions currently used in Ismailia province, Egypt, to control mites. The overall aim of the present study was to study the molecular mechanisms of resistance to the pesticides abamectin and chlorfenapyr in the two-spotted spider mite collected from Ismailia governorate in Egypt. In this paper, we investigated T. urticae collected from sprayed vegetables, fruits and citrus by compounds abamectin and chlorfenapyr where control failures were reported, together with a laboratory strain collected from unsprayed districts. Oligonucleotide specific primers of T. urticae diagnostics were used to screen the genomic DNA as templates based on the end-point PCR analysis. In the present work, searching of T. urticae DNA by PCR for resistance mutations revealed that the amplified DNA fragments had the expected resistant common kinds of mutations. The resulting PCR products were sequenced and compared with the laboratory strain collected from unsprayed districts. Neither mutation was detected in DNA samples of the laboratory strains examined in this study. So, it could be that the toxicological and mutation mechanisms data of abamectin and chlorfenapyr resistance determined in this study will be essential in devising the resistant management strategies for the future use of these compounds in the cultivation in Egypt. | ||||
| Keywords | ||||
| Abamectin; chlorfenapyr; Tetranychus urticae; PCR; resistance; toxicity; spider mite | ||||
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