Assessment of CD4+CD25+ Regulatory T Lymphocytes Cells in Chronic Hepatitis C, Chronic Hepatitis C– Associated Cirrhosis and Hepatocellular Carcinoma Patients
J, M., Abdel-Hai, A., EL din, M., E, A., Mansour, S. (2012). Assessment of CD4+CD25+ Regulatory T Lymphocytes Cells in Chronic Hepatitis C, Chronic Hepatitis C– Associated Cirrhosis and Hepatocellular Carcinoma Patients. EKB Journal Management System, 7(1), 1-7.
Mansour J; Amal Abdel-Hai; Mohamed EL din; Ahmed E; Samira Mansour. "Assessment of CD4+CD25+ Regulatory T Lymphocytes Cells in Chronic Hepatitis C, Chronic Hepatitis C– Associated Cirrhosis and Hepatocellular Carcinoma Patients". EKB Journal Management System, 7, 1, 2012, 1-7.
J, M., Abdel-Hai, A., EL din, M., E, A., Mansour, S. (2012). 'Assessment of CD4+CD25+ Regulatory T Lymphocytes Cells in Chronic Hepatitis C, Chronic Hepatitis C– Associated Cirrhosis and Hepatocellular Carcinoma Patients', EKB Journal Management System, 7(1), pp. 1-7.
J, M., Abdel-Hai, A., EL din, M., E, A., Mansour, S. Assessment of CD4+CD25+ Regulatory T Lymphocytes Cells in Chronic Hepatitis C, Chronic Hepatitis C– Associated Cirrhosis and Hepatocellular Carcinoma Patients. EKB Journal Management System, 2012; 7(1): 1-7.

Assessment of CD4+CD25+ Regulatory T Lymphocytes Cells in Chronic Hepatitis C, Chronic Hepatitis C– Associated Cirrhosis and Hepatocellular Carcinoma Patients

Catrina: The International Journal of Environmental Sciences
Article 1, Volume 7, Issue 1, 2012, Page 1-7  XML PDF (157.14 K)
Document Type: Original Article
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Authors
Mansour J1; Amal Abdel-Hai2; Mohamed EL din3; Ahmed E2; Samira Mansour email orcid 4
1Clinical Laboratory Department, El-Ghad Faculty for Health Science, Jeddah, KSA.
2Department of Clinical Pathology, Faculty of Medicine, Suez Canal University, Ismailia, Egypt.
3Department of Internal Medicine, Faculty of Medicine, Suez Canal University, Ismailia, Egypt.
4Botany Department, Faculty of Science, Suez Canal University, Ismailia, Egypt.
Abstract
CD4+CD25+ regulatory T lymphocytes are a subset of circulating CD4+ T cells with suppressive
properties. CD4+CD25+ regulatory T cells suppress HCV-specific T cell responses and it has been
suggested that they may play a role in viral persistence. Our aim was to assess the frequency of
regulatory T cells in the different clinical presentations of hepatitis C virus infections in a chosen
Egyptian population. Peripheral blood CD4+CD25+ regulatory T cells from patients with different
progression of HCV, was conducted and analyzed using flow cytometry. For each stage of HCV
progression, twenty patients (n=20) were subjected for analysis in comparison with normal healthy
control subjects (n=20). Results showed a significantly higher frequency of CD4+CD25+ regulatory T
cells in chronic HCV (3.0 ± 0.9%), HCV related liver cirrhosis (٣.٣±0.8%), HCV related-HCC (3.9
±1.6%), when compared to normal healthy controls (2.3 ±0.8%). No statistical significant differences
were found when comparing HCV related cirrhotic patients with chronic HCV infected patients
(P=0.95). Meanwhile, statistical significant differences were detected when comparing HCV related
HCC patients with chronic HCV patients (P=0.047). In conclusion, our results indicated the presence of
significant higher than normal frequency of peripheral blood CD4+CD25+ regulatory T cell among
Egyptian patients that may help to early detect the viral disease and open new field to find a way to
disease recovery or protection. Conversely, within different clinical presentation of hepatitis C virus
infection no significant differences were detected. Severity of liver affection was significantly
correlated with viral load.
Keywords
Chronic hepatitis; Flow cytometry; Hepatitis C infection; Hepatitis associated cirrhosis; Hepatocellular carcinoma; liver disease; Regulatory T cells
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