PURIFICATION AND CHARACTERISTICS OF POLYHYDROXY ALKANOATE DEPOLYMERASES FROM EIKENELLA CORRODENS , KLEBSIELLA PNEUMONAE ANDALCALIGENES FAECALIS
Yousef, N., Abd El-Latif, H., Serry, F., Cameron, J. (1998). PURIFICATION AND CHARACTERISTICS OF POLYHYDROXY ALKANOATE DEPOLYMERASES FROM EIKENELLA CORRODENS , KLEBSIELLA PNEUMONAE ANDALCALIGENES FAECALIS. EKB Journal Management System, 7(2), 46-54. doi: 10.21608/zjps.1998.184799
Nehal Yousef; Hemat Abd El-Latif; Fathy Serry; J. A. Cameron. "PURIFICATION AND CHARACTERISTICS OF POLYHYDROXY ALKANOATE DEPOLYMERASES FROM EIKENELLA CORRODENS , KLEBSIELLA PNEUMONAE ANDALCALIGENES FAECALIS". EKB Journal Management System, 7, 2, 1998, 46-54. doi: 10.21608/zjps.1998.184799
Yousef, N., Abd El-Latif, H., Serry, F., Cameron, J. (1998). 'PURIFICATION AND CHARACTERISTICS OF POLYHYDROXY ALKANOATE DEPOLYMERASES FROM EIKENELLA CORRODENS , KLEBSIELLA PNEUMONAE ANDALCALIGENES FAECALIS', EKB Journal Management System, 7(2), pp. 46-54. doi: 10.21608/zjps.1998.184799
Yousef, N., Abd El-Latif, H., Serry, F., Cameron, J. PURIFICATION AND CHARACTERISTICS OF POLYHYDROXY ALKANOATE DEPOLYMERASES FROM EIKENELLA CORRODENS , KLEBSIELLA PNEUMONAE ANDALCALIGENES FAECALIS. EKB Journal Management System, 1998; 7(2): 46-54. doi: 10.21608/zjps.1998.184799

PURIFICATION AND CHARACTERISTICS OF POLYHYDROXY ALKANOATE DEPOLYMERASES FROM EIKENELLA CORRODENS , KLEBSIELLA PNEUMONAE ANDALCALIGENES FAECALIS

Zagazig Journal of Pharmaceutical Sciences
Article 6, Volume 7, Issue 2, December 1998, Page 46-54  XML PDF (2.41 MB)
Document Type: Original Article
DOI: 10.21608/zjps.1998.184799
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Authors
Nehal Yousef email 1; Hemat Abd El-Latif2; Fathy Serry3; J. A. Cameron4
1Department of Microbiology, Faculty of Pharmacy, University of Zagazig - Egypt
2Department of Microbiology and Immunology Faculty of Pharmacy-Zagazig University Zagazig Egypt
3Department of Microbiology and Immuselogy Facelty of Pharmacy-Zagazig University Zagazig Egypt
4Department of Microbiology, Molecular and Cell Biology, Connecticut University , Storrs, U.S.A
Abstract
In a previous screening study for polyhydroxy alkanoate (PHA) degrading activities, three isolates were obtained and characterized as Eikenella corrondens, Klebsiella pneumoniae and Alcaligenes faecalis . In the present study, PHA depolymerases from these three isolates were purified by DEAE anion exchange chromatography and by chromatofocusing ( isoelectric focusing ) after precipitation from the crude enzyme preparations by ammonium sulfate. The molecular weights for the three enzymes were determined by gel permeation sodium dodecyl sulphat (SDS) polyacrylamide gel eclectrophoresis.
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