APPLICATION OF IMMUNOENZYMATIC TECHNIQUE IN DETERMINATION OF GALANTHAMINE IN TWO PANCRATIUM SPECIES | ||||
Zagazig Journal of Pharmaceutical Sciences | ||||
Article 15, Volume 5, Issue 2, December 1996, Page 99-104 PDF (3.16 MB) | ||||
Document Type: Original Article | ||||
DOI: 10.21608/zjps.1996.185026 | ||||
View on SCiNiTO | ||||
Authors | ||||
Taha Sarg 1; Meinhart Zenk2; Sameih El-Dahmy1; Afaf Abdel-Ghani3; Maged Abou-Hashem4 | ||||
1Department of Pharmacognosy, Faculty of Pharmacy, University of Zagazig, Egypt. | ||||
2Institute of Pharmaceutical Biology, Munich University, Munich, Germany | ||||
3Department of Pharmacognosy, Faculty of Pharmacy, University of Zagazig, Zagazig, Egypt. | ||||
4Department of Pharmacognosy, Faculty of Pharmacy, Zagazig University, Zagazig, Egypt | ||||
Abstract | ||||
Enzyme-Linked Immunosorbent Assay (ELISA) was established to qualitatively and quantitatively determine galanthamine in the alcoholic extract of Pancratium foetidum Pomel bulbs and in the callus culture of P. arabicum Sick. The amount of galanthamine was 168 ng/g dry weight of bulbs and 314 ng/g dry weight of the callus. The measuring range for galanthamine extended from 0.1 – 100 ng per assay. The least amount to be detected was 0.01 ng and the recovery was 98 ±1.8 % . | ||||
Statistics Article View: 106 PDF Download: 87 |
||||