Activation Tagging in Aspen Using A Glucocorticoid-Inducible Two Component Ac/Ds-Enhancer Element System
Attalla, H., Zaghloul, M., Waly, A., Fladung, M., El-Sherif, F. (2018). Activation Tagging in Aspen Using A Glucocorticoid-Inducible Two Component Ac/Ds-Enhancer Element System. EKB Journal Management System, 4(2), 45-51.
Hamdy Attalla; Mostafa Zaghloul; Abd El Kawee Waly; Matthias Fladung; Fadia El-Sherif. "Activation Tagging in Aspen Using A Glucocorticoid-Inducible Two Component Ac/Ds-Enhancer Element System". EKB Journal Management System, 4, 2, 2018, 45-51.
Attalla, H., Zaghloul, M., Waly, A., Fladung, M., El-Sherif, F. (2018). 'Activation Tagging in Aspen Using A Glucocorticoid-Inducible Two Component Ac/Ds-Enhancer Element System', EKB Journal Management System, 4(2), pp. 45-51.
Attalla, H., Zaghloul, M., Waly, A., Fladung, M., El-Sherif, F. Activation Tagging in Aspen Using A Glucocorticoid-Inducible Two Component Ac/Ds-Enhancer Element System. EKB Journal Management System, 2018; 4(2): 45-51.

Activation Tagging in Aspen Using A Glucocorticoid-Inducible Two Component Ac/Ds-Enhancer Element System

Catrina: The International Journal of Environmental Sciences
Article 6, Volume 4, Issue 2, 2018, Page 45-51  XML PDF (1.91 MB)
Document Type: Original Article
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Authors
Hamdy Attalla email 1; Mostafa Zaghloul2; Abd El Kawee Waly2; Matthias Fladung3; Fadia El-Sherif2
1Faculty of Agriculture, Suez Canal University. Ismailia
2Department of Horticulture, Faculty of Agriculture, Suez Canal University, Ismailia, Egypt
3Johann Heinrich von Thuenen-Institute (vTI), Federal Research Institute for Rural Areas, Forestry and Fisheries, Institute for Forest Genetics, Sieker Landstr. 2, D-22927 Grosshansdorf, Germany
Abstract
Based on the Ac/Ds two element transposition system from maize an activation tagging approach was
suggested for the hybrid aspen (Populus tremula x tremuloides) line ‘Esch5’. A glucocorticoidinducible
two element Ac/ATDs element system was used to induce activation tagged variants
following two independent transformation steps. In combination with a 35S enhancer tetramer and
outward facing two CaMV 35S promoter located near both ends of the ATDs element, expression of
genes can be elevated located adjacent to the new integration site of the element. As selective marker
for ATDs transposition, knocking-out the expression of a phenotypic marker (rolC gene) was
considered.
Keywords
Activation tagging; glucocorticoid-inducible; rolC gene; Ac/Ds; transposon; ATDs element
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