Activation tagging in aspen using a heat-shock inducible two component Ac/Ds-enhancer element system | ||||
Catrina: The International Journal of Environmental Sciences | ||||
Article 1, Volume 4, Issue 3, 2009, Page 1-7 PDF (342.73 K) | ||||
Document Type: Original Article | ||||
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Authors | ||||
Hamdy Atta-Alla1; Mostafa Zaghloul1; Abd El Kawee Waly1; Matthias Fladung2; Fadia El-Sherif 1 | ||||
1Department of Horticulture, Faculty of Agriculture, Suez Canal University, Ismailia, Egypt | ||||
2Johann Heinrich von Thuenen-Institute (vTI), Federal Research Institute for Rural Areas, Forestry and Fisheries, Institute for Forest Genetics, Sieker Landstr. 2, D-22927 Grosshansdorf, Germany | ||||
Abstract | ||||
From our earlier work on the genetic transfer of the maize transposable element Ac and its functional analysis in hybrid and pure aspen lines, it was shown that this element is active in transgenic aspen. However, a two element transposon tagging system where the Ds element and the transposase gene are put together in crosses is not feasible in trees due to the in part long vegetative phases. To overcome this barrier, an inducible two element HSP-transposase /ATDs element system is suggested to induce activation tagged variants following two independent transformation steps. The ATDs element is comprised by a 35S enhancer tetramer and outwards facing two CaMV 35S promoter located near both ends of the ATDs element. Following transposition of the ATDs element expression of genes can be elevated which are located adjacent to the new integration site of the element. | ||||
Keywords | ||||
Activation tagging; rolC gene; HSP-transposase; ATDs element | ||||
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