Antibiotic susceptibility and capsular genes of Streptococcus pneumoniae colonizing children with chronic respiratory diseases.
Abdallah, F., Fathy, M., Fouda, E., Fahmy, H., Abdallah, N. (2021). Antibiotic susceptibility and capsular genes of Streptococcus pneumoniae colonizing children with chronic respiratory diseases.. EKB Journal Management System, 2(4), 736-747. doi: 10.21608/mid.2021.76739.1167
Fatma Elzahraa Abdallah; Maha Fathy; Eman Fouda; Heba Fahmy; Nermeen Abdallah. "Antibiotic susceptibility and capsular genes of Streptococcus pneumoniae colonizing children with chronic respiratory diseases.". EKB Journal Management System, 2, 4, 2021, 736-747. doi: 10.21608/mid.2021.76739.1167
Abdallah, F., Fathy, M., Fouda, E., Fahmy, H., Abdallah, N. (2021). 'Antibiotic susceptibility and capsular genes of Streptococcus pneumoniae colonizing children with chronic respiratory diseases.', EKB Journal Management System, 2(4), pp. 736-747. doi: 10.21608/mid.2021.76739.1167
Abdallah, F., Fathy, M., Fouda, E., Fahmy, H., Abdallah, N. Antibiotic susceptibility and capsular genes of Streptococcus pneumoniae colonizing children with chronic respiratory diseases.. EKB Journal Management System, 2021; 2(4): 736-747. doi: 10.21608/mid.2021.76739.1167

Antibiotic susceptibility and capsular genes of Streptococcus pneumoniae colonizing children with chronic respiratory diseases.

Microbes and Infectious Diseases
Article 17, Volume 2, Issue 4, November 2021, Page 736-747  XML PDF (454.28 K)
Document Type: Original Article
DOI: 10.21608/mid.2021.76739.1167
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Authors
Fatma Elzahraa Abdallah1; Maha Fathy2; Eman Fouda3; Heba Fahmy4; Nermeen Abdallah email orcid 5
1Microbiology Department, Faculty of Medicine, October 6 University, Egypt
2Medical Microbiology and Immunology Department, Faculty of Medicine, Ain Shams University, Egypt
3Pediatrics department, faculty of Medicine,Ain shams university, Egypt
4Pediatrics Department, Faculty of Medicine, Ain Shams University, Egypt
5Medical Microbiology and Immunology, Faculty of Medicine, Ain shams university, Egypt
Abstract
Background: Streptococcus pneumoniae (S. pneumoniae) is a main pathogen causing acute infectious exacerbations in chronic lung diseases (CLD) children. Determining the local antibiotic susceptibility pattern of colonizing strains in these patients is crucial for empirical therapy. Moreover, identifying prevalent types is important to evaluate the effectiveness of available vaccines.
This study aimed to detect antibiotic susceptibility and searching for some capsular gene types among S. pneumoniae isolates colonizing respiratory tract in CLD children.
Methods: Bronchoalveolar lavage (BAL) samples were collected from 51 CLD children undergoing bronchoscopy in Ain Shams University Pediatric Hospital. All identified S. pneumoniae isolates were tested for antibiotic susceptibility. Two-steps sequential multiplex PCR technique was performed to detect capsular gene types: [3/ 22f/(22A) / 19A / 6A/6B /4 / 14/ 12F/(12A) / 9V/(9A).
Results: From 51 BAL samples, 32 (62.75%) pneumococcal strains were isolated. Most of the isolates had capsular gene type 6A/6B (65.6%). Capsular gene type 14 was detected in 25% of isolates. In 9.4% of strains, capsular type could not be identified. All isolates were sensitive to vancomycin. The lowest resistance rate was to levofloxacin (6.3%) and linezolid (9.4%), while the highest rates were to clindamycin (71.9%) and erythromycin (68.8%).
Conclusion: S. pneumoniae colonizing CLD children showed high resistance to clindamycin and erythromycin thus highlighting the importance of antimicrobial stewardship programs in all levels of healthcare. Capsular gene type 6A/6B was the commonest colonizing type suggesting that CLD children can benefit from the currently available PCV13 vaccine.
Keywords
Streptococcus pneumoniae; Chronic Lung Diseases; Capsular Gene
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