Molecular Identification of Egyptian Eggplant Cultivars (Solanum melongena L.) Using RAPD and RFLP of 18S rRNA Gene Markers
Abdel-Razzak, H., AbdAl-Aziz, S., Hafez, E. (2008). Molecular Identification of Egyptian Eggplant Cultivars (Solanum melongena L.) Using RAPD and RFLP of 18S rRNA Gene Markers. EKB Journal Management System, 3(3), 1-10.
Hesham Abdel-Razzak; Samia AbdAl-Aziz; Elsayed Hafez. "Molecular Identification of Egyptian Eggplant Cultivars (Solanum melongena L.) Using RAPD and RFLP of 18S rRNA Gene Markers". EKB Journal Management System, 3, 3, 2008, 1-10.
Abdel-Razzak, H., AbdAl-Aziz, S., Hafez, E. (2008). 'Molecular Identification of Egyptian Eggplant Cultivars (Solanum melongena L.) Using RAPD and RFLP of 18S rRNA Gene Markers', EKB Journal Management System, 3(3), pp. 1-10.
Abdel-Razzak, H., AbdAl-Aziz, S., Hafez, E. Molecular Identification of Egyptian Eggplant Cultivars (Solanum melongena L.) Using RAPD and RFLP of 18S rRNA Gene Markers. EKB Journal Management System, 2008; 3(3): 1-10.

Molecular Identification of Egyptian Eggplant Cultivars (Solanum melongena L.) Using RAPD and RFLP of 18S rRNA Gene Markers

Catrina: The International Journal of Environmental Sciences
Article 1, Volume 3, Issue 3, 2008, Page 1-10  XML PDF (964.29 K)
Document Type: Original Article
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Authors
Hesham Abdel-Razzak email 1; Samia AbdAl-Aziz2; Elsayed Hafez3
1Vegetable Crops Department, Faculty of Agriculture, Alexandria University, Alexandria, Egypt
2Nucleic Acid Research Department, Genetic Engineering and Biotechnology Research Institute, Mubarak City for Scientific Research and Technology Application, Alexandria, Egypt
3Arid Lands Research Institute, Plant Molecular Pathology Department, Borg El Arab City, Alexandria, Egypt
Abstract
Seven Eggplant (Solanum melongena L.) genotypes, representing the three common Egyptian local
cultivars, were subjected to random amplified polymorphic DNA (RAPD) analysis using ten random
primers. The ten primers generated 657 RAPD fragments, of which 77 were polymorphic with 11.72 %.
The seven genotypes were discriminated with 3-11 distinct polymorphic bands generated from the
primers CHI 15 (3), and RAP, EZ and NAH (11). The genetic similarity was estimated for each primer
among the seven genotypes. Results of a cluster analysis showed that the genetic similarity between the
seven genotypes ranged from 58.0% to 92.0%. The highest genetic similarity of 92.0% was detected
between genotypes LB1 and LB2 (original population "OP" and selected population "SP"; long slender
black fruits), followed by 86.0% between genotypes RB1 and RB2 (OP and SP; round or egg shaped
black fruits), and 80.0% between genotypes LB2 (SP; long slender black fruits) and RB2 (SP; round or
egg shaped black fruits). However, the lowest genetic similarity of 58.0% was detected between both
genotypes LW1 and LW2 (OP and SP; long slender white fruits) and genotype PO (SP; purple oblong
shape fruits), followed by RB1 (OP; round or egg shaped black fruits) and PO (61.0%). Restriction
fragment length polymorphism (RFLP) of 18S rRNA gene was carried out using four different restriction
enzymes viz. EcoRI, TaqI, PstI and HindIII. Only the restriction pattern of Taq I enzyme showed distinct
polymorphic bands among the seven examined eggplant genotypes and divided them into three different
groups. However, the RAPD-PCR succeeded to divide them into two major groups; group one contained
only genotype PO (SP; purple oblong shape fruits) and the second group included the other genotypes.
Keywords
Eggplant; Genetic diversity; long slender white and black fruits; RAPD and RFLP of 18S rRNA PCR techniques
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