In Vivo Assessment of the Antischistosomal Activity of OPUNTIA FICUS-INDICA Flowers and Their Chemical Constituents
SHEDED, A., EL-HASHASH, M., eltomy, S., Salib, J., William, S., Hamed, M. (2021). In Vivo Assessment of the Antischistosomal Activity of OPUNTIA FICUS-INDICA Flowers and Their Chemical Constituents. EKB Journal Management System, 64(8), 4557-4568. doi: 10.21608/ejchem.2021.77763.3809
Asmaa H. SHEDED; Maher A. EL-HASHASH; sayed abdelhamid eltomy; Josline Y. Salib; Samia William; Manal Mortady Hamed. "In Vivo Assessment of the Antischistosomal Activity of OPUNTIA FICUS-INDICA Flowers and Their Chemical Constituents". EKB Journal Management System, 64, 8, 2021, 4557-4568. doi: 10.21608/ejchem.2021.77763.3809
SHEDED, A., EL-HASHASH, M., eltomy, S., Salib, J., William, S., Hamed, M. (2021). 'In Vivo Assessment of the Antischistosomal Activity of OPUNTIA FICUS-INDICA Flowers and Their Chemical Constituents', EKB Journal Management System, 64(8), pp. 4557-4568. doi: 10.21608/ejchem.2021.77763.3809
SHEDED, A., EL-HASHASH, M., eltomy, S., Salib, J., William, S., Hamed, M. In Vivo Assessment of the Antischistosomal Activity of OPUNTIA FICUS-INDICA Flowers and Their Chemical Constituents. EKB Journal Management System, 2021; 64(8): 4557-4568. doi: 10.21608/ejchem.2021.77763.3809

In Vivo Assessment of the Antischistosomal Activity of OPUNTIA FICUS-INDICA Flowers and Their Chemical Constituents

Egyptian Journal of Chemistry
Article 53, Volume 64, Issue 8, August 2021, Page 4557-4568  XML PDF (712.87 K)
Document Type: Original Article
DOI: 10.21608/ejchem.2021.77763.3809
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Authors
Asmaa H. SHEDEDorcid 1; Maher A. EL-HASHASH1; sayed abdelhamid eltomyorcid 2; Josline Y. Salib email orcid 3; Samia William4; Manal Mortady Hamedorcid 5
1Organic Chemistry Department, Faculty of Science, Ain-Shams University
2National Research centre Tanning lab.
3Chemistry of Tannins, National Research Center, Cairo, Egypt.
4Parasitology Department, Theodor Bilharz Research Institute, Ministry of Scientific Research
5Medicinal Chemistry, Theodor Bilharz Research Institute, Giza, Egypt
Abstract
Hepatic schistosomiasis is the most well-known form of chronic disease, with a wide variety of clinical symptoms. The aim of this study was to assess the schistosomicidal effect of the aqueous methanolic extract of Opuntia ficus-indica flowers in-vivo regarding disease progression in a comparative experimental study to praziquantel (PZQ). Phytochemical investigation of flowers extract resulted in thirteen phenolic and flavonoid compounds. Structures of these compounds were elucidated by UV and 1D⁄2D 1H⁄ 13C NMR spectroscopy and compared with the literature data. From the 49th day after infection, mice infected with Schistosoma mansoni were given the extract (200 mg/kg) orally every day for 5 days, whereby the Praziquantel (500 mg/kg) was used as reference drug. The parasitological parameters (total worm burden, tissue egg load and oogram pattern) per gram of liver or intestinal tissue of the infected and the treated mice were counted. A histopathological examination of the liver granuloma took place as well. Opuntia ficus-indica extract caused a substantial decrease in the number of worms and eggs (%); the extract also decreased worm and egg burdens moderately. The results demonstrated the flower extract as a promising antischistosomal activity due to the parasitological and histopathological changes induced, besides it highlights the importance of its polyphenolic constituents.
Keywords
Opuntia ficus-indica; flowers; flavonoids constituents; antischistosomal activity
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