COMPARATIVE STUDY ON ELECTROPHORETIC PROTEIN PATTERN CHARACTERIZATION OF TILAPIA SPECIES IN THE RIVER NILE, EGYPT. | ||||
Egyptian Journal of Aquatic Biology and Fisheries | ||||
Article 8, Volume 10, Issue 2, April 2006, Page 147-178 PDF (2.3 MB) | ||||
Document Type: Original Article | ||||
DOI: 10.21608/ejabf.2006.1856 | ||||
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Authors | ||||
Sabry El-Serafy; Nassr-AHah Abdel- Hamide; Mona Azab; Mohammed Awwad | ||||
Department of Zoology, Faculty of Science, Benha University, Benha, Egypt. | ||||
Abstract | ||||
This study deals with the electrophoretic characterization of Tilapia species using plasma and muscle proteins, as a taxonomically tool to differentiate Tilapia species that live in Egyptian habitat of the River Nile. These species are Oreochromis nilotrcits ( O. nihticus ) , O. aureus fiarotherodon galilaeits ( S. galilaeus ) and Tilapia zillii ( T. zillii). The data of electrophoretic protein separation indicated that each species has a characteristic specific pattern with more common bands for all species as well as species specific bands characteristic for each one. In this study, the protein of plasma and muscle were examined either treated with sample buffer or not treated. The results indicated that the protein pattern among the closer species exhibits common characters. In this regard, the obtained protein pattern of O. niloticus looks like that of O. aureus indicating a monophylogenetic origin of these species. Whereas, less degree of similarity was recorded between T. zillii and other species, indicating a genetic distance between this species and the rest of Tilapia species. | ||||
Keywords | ||||
Tilapia species; protein electrophoresis; plasma; muscle; monophylogenetic. River Nile | ||||
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