The protective role of Diosmin, Hesperidine combination against heavy metals toxicity in Wistar albino rats: Biochemical, Immunohistochemical and Molecular Studies | ||||
Egyptian Journal of Chemistry | ||||
Article 51, Volume 64, Issue 8, August 2021, Page 4531-4543 PDF (1.02 MB) | ||||
Document Type: Original Article | ||||
DOI: 10.21608/ejchem.2021.83002.4078 | ||||
View on SCiNiTO | ||||
Authors | ||||
Hazem Kamel Sarhan 1; Ahmed M.A. Saleh2; Olfat Hammam3; Aly H Atta4; Eslam Elnahrery4 | ||||
1Medical and Radiation department, Research sector, Nuclear Material Authority | ||||
2Head of Giza scientific office, Egyphar for Pharmaceutical Industry, El-Obbore, Cairo, Egypt | ||||
3Theodor Bilharze institute | ||||
4Chemistry Department, Faculty of Science, Suez University, Suez, Egypt | ||||
Abstract | ||||
The benchmark of this study is to evaluate the antioxidant protective efficiency of Diosmin-Hesperidin combination, a natural citrus flavone of hesperidin derivative on heavy metals intoxication and Oxidative stress-induced damage in Wistar albino rats. Oral doses of diosmin-hesperidin in rats (200 and 100 mg/kg body weight, respectively) for a month (every other day) prior to heavy metals intoxication. Evaluation of the protective and antioxidant effects of the combination of diosmin and hesperidin, various Rt-PCR estimations, biochemical estimations, histopathological alterations as well as comet assay and caspase-3 activity for assessment of apoptosis were performed. Results indicated that heavy metals intoxication-induced decline in the levels of liver tissue P53 gene expression and increase of liver tissues of the apoptotic caspase-3 gene, also induced decline in the levels of liver tissue antioxidant parameters (SOD, GPx, and GSH), increased lipid peroxidation (MDA), DNA damage and apoptosis, these parameters were improved by pre-administration of diosmin+hesperidine. Diosmin+hesperidine dose (200 and100 mg/kg body wt. respectively) restored the p53 and caspase-3 genes near-normal values, antioxidant status to near normal and reduced lipid peroxidation, DNA, and tissue damage. These results were confirmed by histopathological examinations, which showed that pre-administration of diosmin+hesperidine protected the liver of albino rats against heavy metals intoxication-induced damage. Hence, it has been illustrated that diosmin+hesperidine might be an effective antioxidant and protector against heavy metals intoxication-induced damage in rats. Moreover, the diosmin+hesperidine alone pretreated group did not show any biochemical alterations, fold change of P53 or caspase-3 genes or DNA damage indicating the protective nature of the drug. | ||||
Keywords | ||||
Heavy metals; Diosmin+Hesperidine; Antioxidants; Rt-PCR; DNA damage; Apoptosis; Histopathology | ||||
Statistics Article View: 652 PDF Download: 410 |
||||