Long-term Stability of Two Rauwolfia serpentina Cell Strains
Andreev, I., Adnof, D., Spiridonova, K., Kunakh, V. (2007). Long-term Stability of Two Rauwolfia serpentina Cell Strains. EKB Journal Management System, 2(2), 133-136.
Igor Andreev; Daniel Adnof; Katerina Spiridonova; Viktor Kunakh. "Long-term Stability of Two Rauwolfia serpentina Cell Strains". EKB Journal Management System, 2, 2, 2007, 133-136.
Andreev, I., Adnof, D., Spiridonova, K., Kunakh, V. (2007). 'Long-term Stability of Two Rauwolfia serpentina Cell Strains', EKB Journal Management System, 2(2), pp. 133-136.
Andreev, I., Adnof, D., Spiridonova, K., Kunakh, V. Long-term Stability of Two Rauwolfia serpentina Cell Strains. EKB Journal Management System, 2007; 2(2): 133-136.

Long-term Stability of Two Rauwolfia serpentina Cell Strains

Catrina: The International Journal of Environmental Sciences
Article 5, Volume 2, Issue 2, 2007, Page 133-136  XML PDF (1.03 MB)
Document Type: Original Article
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Authors
Igor Andreev email ; Daniel Adnof; Katerina Spiridonova; Viktor Kunakh
Institute of Molecular Biology & Genetics, National Academy of Sciences of Ukraine, Str. Acad. Zabolotnogo, 150, Kiev-143, 03143, Ukraine
Abstract
In these latter days there is ever growing demand for medicinal drugs and nutrient supplements of natural
origin, in particular derived from plant raw material. At the same time, exhaustion of natural resources
has risen the problem of search for new alternative sources of plant raw material. Cell biotechnology may
propose the way out of this situation. Development of the techniques for maintenance of the plant cells,
tissues and organs under controlled conditions on the artificial nutrient media provides the possibility for
production of desirable amounts of ecologically clean plant biomass. In this work we investigated the
long-term genetic stability of plant tissue culture, which is prerequisite for its durable use for industrial
production of plant based drugs. Genetic analysis of Rauwolfia serpentina Benth. cultured tissues from
two strains to be sampled with an interval of time more than 10 years was carried out through RAPDPCR.
The genome of cultured plant cells was found to lack of obvious changes during long-term
maintenance. The data obtained evidence for significant stability of the tissue culture under growth in
standard condition. Used RAPD-markers seem to allow differentiate various strains of cultured R.
serpentina tissues.
Keywords
Rauwolfia serpentina; plant tissue culture; in Vitro; cell line K-20; cell line K-27; indoline alkaloids production; ajmaline; genetic polymorphism; RAPD-PCR; plant raw material
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