STUDIES ON THE VACCINAL STRAIN OF REOVIRUS
HASSAN, N., ABOU EL-KHAIR, M., EL-BORDENY, F., EL-ASSILY, S., KHASHABAH, E., WAHBA, S., SABER, M. (1993). STUDIES ON THE VACCINAL STRAIN OF REOVIRUS. EKB Journal Management System, 29.1(57), 50-61. doi: 10.21608/avmj.1993.186327
NADIA M. HASSAN; M. ABOU EL-KHAIR; FEKRIA EL-BORDENY; SALWA EL-ASSILY; ENSAF M. KHASHABAH; S. WAHBA; M.S. SABER. "STUDIES ON THE VACCINAL STRAIN OF REOVIRUS". EKB Journal Management System, 29.1, 57, 1993, 50-61. doi: 10.21608/avmj.1993.186327
HASSAN, N., ABOU EL-KHAIR, M., EL-BORDENY, F., EL-ASSILY, S., KHASHABAH, E., WAHBA, S., SABER, M. (1993). 'STUDIES ON THE VACCINAL STRAIN OF REOVIRUS', EKB Journal Management System, 29.1(57), pp. 50-61. doi: 10.21608/avmj.1993.186327
HASSAN, N., ABOU EL-KHAIR, M., EL-BORDENY, F., EL-ASSILY, S., KHASHABAH, E., WAHBA, S., SABER, M. STUDIES ON THE VACCINAL STRAIN OF REOVIRUS. EKB Journal Management System, 1993; 29.1(57): 50-61. doi: 10.21608/avmj.1993.186327

STUDIES ON THE VACCINAL STRAIN OF REOVIRUS

Assiut Veterinary Medical Journal
Article 6, Volume 29.1, Issue 57, April 1993, Page 50-61  XML PDF (3.25 MB)
Document Type: Research article
DOI: 10.21608/avmj.1993.186327
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Authors
NADIA M. HASSAN; M. ABOU EL-KHAIR; FEKRIA EL-BORDENY; SALWA EL-ASSILY; ENSAF M. KHASHABAH; S. WAHBA; M.S. SABER
Abstract
The present study was concerned with the detection of the most suitable condition for propagation and preservation of vaccinal strain of Reovirus. The most suitable route of inoculation for embryonated chicken eggs (ECE) was chorio-allantoic membrane (CAM) and allantoic cavity (AC) giving an infective titer 109 and 10' */ ml EID50 respectively. The best dilution of Reovirus used for egg inoculation was 10. Determination of the best time for harvesting the virus was the fourth day post inoculation. The suitable organ forvirus multiplication was embryo and mixture of embryo plus CAM and AAF giving a titer of 100*44 and EID50/ml respectively. Studies on the effect of using different stabilizers on the EID50 of lyophilized virus showed that skimmed milk gave the highest EID50 (10'.*/ml). Stability of Reovirus at 56°C for 8 hours.
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