Assessment of Egyptian Maize Hybrids for Their Capability of Plant Regeneration using Immature Embryo Culture | ||||
| Alexandria Science Exchange Journal | ||||
| Article 4, Volume 42, Issue 3, September 2021, Page 619-627 PDF (387.03 K) | ||||
| Document Type: Original Article | ||||
| DOI: 10.21608/asejaiqjsae.2021.186822 | ||||
 View on SCiNiTO | ||||
| Authors | ||||
Shaimaa M. Awad-Allah* ; Yohana Kuku* ; Sanaa I. Milad* ; M. N. Barakat 
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| Biotechnology Laboratory, Crop Science Department,Faculty of Agriculture, University of Alexandria, P.O. Box 21545, Alexandria, Egypt | ||||
| Abstract | ||||
| The aim of this research was to determine the regeneration capability of five maize hybrids (Syngenta hybrid, Three-way hybrid (324), Single cross hybrid P344 (Pioneer), Single cross hybrid 30N 11 (Pioneer) and Three-way hybrid (321)) using immature embryo explants with four medium protocols. The analysis of variance for in vitro culture traits of explant response indicated that callus induction, callus weight and embryogenic callus significantly responded to maize genotypes, medium protocols and their interaction. Also differences among maize hybrids were highly significant for shoot formation (%), while they were not significant for root formation (%). The four medium protocols indicated highly significant differences for root formation (%) and shoot formation (%). Three-way hybrid (324) and Single cross hybrid 30 N11(Pioneer) had the highest induction frequency of shoot formation on medium protocol D. Both maizehybrids on medium protocol D could be successfully utilized for the in vitro selection studies for maize improvement. | ||||
| Keywords | ||||
| Maize; Immature embryo; Tissue culture; Plant regeneration | ||||
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