Detection of colistin resistant E. coli in children at pediatric hospital of Assiut University, using phenotypic and genotypic Methods
Ghandour, A., Sror, S., Sabet, M., Rashwan, R. (2021). Detection of colistin resistant E. coli in children at pediatric hospital of Assiut University, using phenotypic and genotypic Methods. EKB Journal Management System, 2(4), 748-759. doi: 10.21608/mid.2021.82048.1165
Aliaa Ghandour; Shaban Sror; Marwa Sabet; Ragaa Rashwan. "Detection of colistin resistant E. coli in children at pediatric hospital of Assiut University, using phenotypic and genotypic Methods". EKB Journal Management System, 2, 4, 2021, 748-759. doi: 10.21608/mid.2021.82048.1165
Ghandour, A., Sror, S., Sabet, M., Rashwan, R. (2021). 'Detection of colistin resistant E. coli in children at pediatric hospital of Assiut University, using phenotypic and genotypic Methods', EKB Journal Management System, 2(4), pp. 748-759. doi: 10.21608/mid.2021.82048.1165
Ghandour, A., Sror, S., Sabet, M., Rashwan, R. Detection of colistin resistant E. coli in children at pediatric hospital of Assiut University, using phenotypic and genotypic Methods. EKB Journal Management System, 2021; 2(4): 748-759. doi: 10.21608/mid.2021.82048.1165

Detection of colistin resistant E. coli in children at pediatric hospital of Assiut University, using phenotypic and genotypic Methods

Microbes and Infectious Diseases
Article 18, Volume 2, Issue 4, November 2021, Page 748-759  XML PDF (661.04 K)
Document Type: Original Article
DOI: 10.21608/mid.2021.82048.1165
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Authors
Aliaa Ghandour email 1; Shaban Sror2; Marwa Sabet3; Ragaa Rashwan1
1Medical Microbiology and Immunology Department, Faculty of Medicine, Assiut University, Assiut, Egypt
2Gastroenterology and Hepatology Unit, Pediatric Department, Faculty of Medicine, Assiut University, Assiut, Egypt.
3Department of Microbiology and Immunology, Faculty of Pharmacy, Sphinx University, New-Assiut, Egypt
Abstract
Background: The emergence of colistin-resistant strains is considered a great threat for the children suffering from diarrhea. This study aimed to screen for the presence of mcr-1 in Escherichia coli (E. coli) isolates collected from children with diarrhea and to compare between genotypic and phenotypic methods for detection of colisitin resistant E.coli carrying mcr-1gene. Methods: Isolation of E.coli was done followed by antimicrobial susceptibility test. Kirby-Baur disc diffusion was used to determine antimicrobial susceptibility, whereas broth microdilution (BMD) and the double disc synergy test (DDST) were used to determine colistin resistance. The screening for mcr-1 was used to investigate one probable mechanism of colistin resistance by PCR. Results: All mcr-1 E.coli isolates were resistant to ampicillin, while resistance to ampicillin/sulbactam, cefazolin, cefoxitin, ceftazidime and trimethoprim-sulphamethoxazol was 94.1% (32/34), 94.1% (32/34), 94.1% (32/34), 85.3% (29/34) and 70.6% (24/34) respectively. All mcr-1carrying E. coli strains were sensitive to tobramycin, amikacin and imipenem. Moderate resistance was noticed to piperacillin/ tazobactam(23/34) 67.6%, gentamycin 47.1% (16/34), and ciprofloxacin 44.1% (15/34). Thirty-one (91.2 %) mcr-1 positiveE. coli strains were multidrug resistant (MDR). Forty five out of 95 (47.4%) of E.coli isolates were positive for mcr-1 by DDST and 34 /95 (35.78%) of E. coli isolates were positive for mcr-1 by PCR. Conclusions: This study reported a high prevalence of colistin resistant E. coli harboring mcr-1 gene in young children in Pediatric Hospital of Assiut University. Broth microdilution is more accurate than DDST in detection of colistin resistance.
Keywords
E. coli; colistin resistance; mcr-1
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