Effect of Separation Methods on Identification and Determination of Concentrations the Bioactive Compounds | ||||
| Journal of Environmental Studies | ||||
| Article 4, Volume 8, Issue 1, June 2012, Page 25-29 PDF (336.63 K) | ||||
| Document Type: High quality original papers | ||||
| DOI: 10.21608/jesj.2012.189169 | ||||
 View on SCiNiTO | ||||
| Authors | ||||
Sagida A. Hussain1; Muna Mahmud1; Zainab T. Al-Sharify  2; Muna A. Jaffar1; Alia Hussain1
| ||||
| 1Ministry of science and technology, Baghdad, Iraq. | ||||
| 2Environmental Engineering Department, College of Engineering, Al-Mustansiryiah University, Bab AL- Muthem, Baghdad, Iraq. | ||||
| Abstract | ||||
| The objective of this study was to use different methods to isolate and separate the bioactive compounds found in leaves and seeds of Datura metel and these methods include solvent extraction by chloroform and chromatography separation by sorbent material such as silica gel (Merck Column Extrelut®1), as well as to study effect of deffating operation from seeds in separation of alkaloids. Results showed that weight percentage of alkaloids were 0.15, 0.36, 0.60 and 0.82% for seeds and leaves of Datura metel by solvent extraction and column chromatography respectively. High Performance Liquid Chromatographic (HPLC) was employed to analysis the extracts which proved that, the chromatography separation of alkaloids was more accurate and active than solvent extraction. | ||||
| Keywords | ||||
| Extraction; Datura metel; Alkaloids; Merck Column Extrelut®1 | ||||
|
Statistics Article View: 88 PDF Download: 100 |
||||
