ISOLATION AND MOLECULAR CHARACTERIZATION OF POTENTIAL CELLULOLYTIC BACILLUS SPECIES ISOLATED FROM SOIL SAMPLES | ||||
| Fayoum Journal of Agricultural Research and Development | ||||
| Article 1, Volume 34, Issue 2, July 2020, Page 1-12 PDF (944.2 K) | ||||
| Document Type: Research articles. | ||||
| DOI: 10.21608/fjard.2020.189747 | ||||
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| Authors | ||||
Esraa Abd Elhameed1; Gamal Hassan  2; Alaa Ropy3; Tharwat El Desoky4
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| 11Chemistry department, Faculty of Science, Fayoum University, Fayoum, 63514, Egypt | ||||
| 2Genetics department, Faculty of Agriculture, Fayoum University, Fayoum,63514, Egypt. | ||||
| 3Chemistry department, Faculty of Science, Fayoum University, Fayoum, 63514, Egypt | ||||
| 4Botany department, Faculty of Science, Fayoum University, Fayoum, 63514, Egypt. | ||||
| Abstract | ||||
| The twenty-two bacterial isolates were screened for cellulolytic activity using Congo red stain on Carboxymethylcellulose (CMC) agar plates inoculated with the isolates. All the isolates were found to hydrolyze Carboxymethylcellulose. Five bacterial isolates (FAY88, FAY103, FAY117. FAY136 and FAY182) were selected according to their higher production of carboxymethyl cellulase (CMCase). A Gram stain test was carried out to identify five isolates as Gram-positive rods, all associated mainly with members of the Bacillus. Their CMCase activities were 1.49, 1.26, 1.21, 1.21 and 1.24 U/ml, respectively. Genetic distances for the five selected strains with high cellulase activity based on the 79 bands obtained from the results of RAPD revealed the similarity according to the morphological characterization agrees with genetic distance. The SDS-PAGE analysis was revealed that, the molecular weight of crude cellulase isolated from Bacillus isolates was found approximately of 54 kDa. | ||||
| Keywords | ||||
| Bacillus; Cellulase activity; morphological characterization; RAPDPCR | ||||
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