The Possible Curative Role of Bone Marrow-Derived Mesenchymal Stem Cells in Bleomycin-Induced Skin Changes in Adult Male Albino Rats | ||
| Egyptian Journal of Histology | ||
| Article 12, Volume 45, Issue 4, December 2022, Pages 1125-1145 PDF (2.64 M) | ||
| Document Type: Original Article | ||
| DOI: 10.21608/ejh.2021.80788.1505 | ||
| Authors | ||
| Amal Abd-Elhafez1; Maram Elkelany2; Amany Mousa2; Thorya El-deeb3; Amira Adly Kassab* 4 | ||
| 1Histology Department, Faculty of Medicine, Tanta University | ||
| 2Histology Department Faculty of Medicine Tanta University | ||
| 3Histology Department,Faculty of Medicine Tanta University | ||
| 4Histology and Cell Biology Department, Faculty of Medicine,Tanta University, Egypt | ||
| Abstract | ||
| Introduction: Bleomycin is a chemotherapeutic agent that has been applied in the treatment of many tumors. Its application results in various cutaneous side effects. The role of bone marrow-derived mesenchymal stem cells [BM-MSCs] has been proved in tissue regeneration. Aim of the Work: Study of the effect of bleomycin on the histological structure of the skin of adult male albino rats and evaluation of the possible curative role of BM-MSCs. Materials and Methods: Forty-five adult male albino rats were divided into two groups. Control group included 15 rats. Experimental group included 30 rats which were subdivided into three equal subgroups: 2A, 2B & 2C. Rats of the experimental group were injected with bleomycin subcutaneously for four weeks. Each rat of subgroup 2A received [0.1mg] of bleomycin. Each rat of subgroup 2B received [1ml] of the culture media of the bone marrow cells one day after the last bleomycin dose. Each rat of subgroup 2C received BM-MSCs (2.5×106) one day after the last bleomycin dose. Skin specimens were examined by the light and electron microscopy. Immunohistochemical study was done by using antibodies to transforming growth factor beta (TGF-β). Evaluation of the collagen surface area percentage and the number of TGF-β positive cells was performed. Results: Experimental subgroups 2A & 2B showed the disturbance of the normal architecture of the skin. There was a significant increase in the surface area percentage of collagen and number of TGF-β positive cells. Ultrastructurally, some keratinocytes showed cytoplasmic vacuolation, swollen disrupted mitochondria and nuclear changes. In contrast, the experimental subgroup 2C showed minimal structural changes and a non-significant difference in all mentioned parameters. Conclusion: Bleomycin altered the normal structure of the skin of adult male albino rats. Bone marrow-derived MSC improved the skin changes induced by bleomycin. | ||
| Keywords | ||
| Bleomycin; BM-MSCs; electron microscopy; immunohistochemistry; skin | ||
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