Protective Effects of Pomegranate Peel Extract on the Diabetic –Induced Damage in Rat Testis | ||||
| Egyptian Journal of Histology | ||||
| Article 20, Volume 45, Issue 4, December 2022, Page 1256-1269 PDF (13.51 MB) | ||||
| Document Type: Original Article | ||||
| DOI: 10.21608/ejh.2021.83818.1518 | ||||
 View on SCiNiTO | ||||
| Author | ||||
Amgad Gaber Elsaid  
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| anatomy, faculty of medicine, Ain shams university, Cairo, Egypt | ||||
| Abstract | ||||
| Introduction: Diabetes mellitus (DM) is known to cause a state of general oxidative stress. The decreased fertility rates have been attributed to testicular inflammation, oxidative stress and apoptosis in male diabetic patients. Pomegranate peel extract (PPE), known for its high content of bioactive constituents, posses anti-inflammatory, antioxidant, antidiabetic properties. Aim of the Work: To investigate the potential benefits of PPE against testicular injury-induced by diabetes. Material and Methods: Forty adult male albino rats were randomly allocated to four groups: control group, PPE group (500 mg/kg/day orally for 8 weeks), diabetic group (received single intraperitoneal injection of 60 mg/kg STZ) and diabetic treated with PPE group. At the end of the experiment, the testes were dissected out and processed for light microscopy, immunohistochemical stainning and transmission electron microscope. Serum testosterone hormone were investigated. Results: Examination of diabetic group revealed a significant reduced serum testosterone level, severely damaged testis with thickened tunica albuginea, thickening and discontinuity of the tubular basement membrane, shrunken tubules with occluded lumen, depleted germ cells, markedly lost elongated spermatids, congestion and significantly increased caspase-3 immunopositive cells area percent. TEM results revealed shrunken nuclei, ballooned mitochondria, cytoplasmic vacuolations and wide intercellular spaces. Diabetic treated with PPE group showed restoration of normal arrangement of the seminiferous tubules with lumen full with mature elongated spermatids and narrow interstitial spaces in-between. Conclusion: DM induced damaging effect on the testis, increased the expression of caspas-3 apoptotic marker, fibrosis and lowered the testosterone level. However, PPE administration counteracted most of these changes so it could be used as adjuvant therapy for DM. | ||||
| Keywords | ||||
| Diabetes mellitus; pomegranate peel extract; testis, TEM | ||||
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