Evaluation of nested PCR for diagnosis of Cyclospora cayetanensis in a sample of immunosuppressed and diarrheic patients in Turkey | ||||
| Parasitologists United Journal | ||||
| Article 5, Volume 14, Issue 2, August 2021, Page 141-145 PDF (477.47 K) | ||||
| Document Type: Original Article | ||||
| DOI: 10.21608/PUJ.2021.55459.1101 | ||||
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| Authors | ||||
| Abdurrahman Ekici* 1; Ahmet Unlu2; Hasan Yilmaz3; Zeynep Cengiz3; Yunus Beyhan3 | ||||
| 1Department of Parasitology, Faculty of Medicine1, | ||||
| 2Gevaş Vocational School, Veterinary Division | ||||
| 3Department of Parasitology, Faculty of Medicine | ||||
| Abstract | ||||
| Background: Cyclospora cayetanensis is a food-borne coccidian parasite that causes cyclosporiasis in humans and possibly in other animals. It presents with watery diarrhea and other related symptoms. Since detection of oocysts may be difficult with histological stains, a negative result should not exclude the possibility of C. cayetanensis. PCR methods can achieve more sensitive detection of the parasite. Objective: The presence of C. cayetanensis was investigated in an immunosuppressed patient group, diarrhea patient group, and in both immunosuppressed and diarrhea patient group using the modified acid-fast staining and nested polymerase chain reaction (nPCR) methods. Subjects and Methods: Included in the study were 80 patients with immune suppression, 50 patients with diarrhea, and 70 patients with both immune suppression and diarrhea. The clinical findings of these patients were recorded, stool samples were collected and examined using both the modified acid-fast (AF) staining and nPCR methods. Results: The overall detection rate of C. cayetanensis was 8% and 12% using the modified AF and nPCR, respectively. C. cayetanensis was detected in 5% of immunosuppressed patients, 12%, in patients with diarrhea and 20% in patients with both immune suppression and diarrhea. Statistically significant relationships were identified between the frequency of C. cayetanensis and abdominal pain (P<0.01), nausea (P<0.01), fatigue (P<0.01), diarrhea (P<0.05), and weight loss (P<0.01). Conclusion: nPCR gave a higher rate of cyclosporiasis, and it is more appropriate especially in cases with recurrent prolonged symptoms. | ||||
| Keywords | ||||
| Cyclospora cayetanensis; diarrhea; immune suppressed; modified acid-fast staining; nested PCR | ||||
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