ANTIOXIDANT, ANTICANCER AND IMMUNOCYTOCHEMISTRY ACTIVITIES OF THREE SYNTHETIC COUMARIN DERIVATIVES | ||||
Fayoum Journal of Agricultural Research and Development | ||||
Article 7, Volume 31, Issue 2, July 2017, Page 79-94 PDF (1.91 MB) | ||||
Document Type: Research articles. | ||||
DOI: 10.21608/fjard.2017.191254 | ||||
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Authors | ||||
Amr E. M. Mahmoud1; Fasil A. Abebe2; Ghada O. El-Demerdash3; Ekkehard A. Sinn4 | ||||
1Biochemistry Department, Faculty of Agriculture, Fayoum University, Egypt. | ||||
2Chemistry Department, Morgan State university, USA | ||||
3Animal Health Research Institute, Agricultural Research Center, Egypt. | ||||
4Chemistry Department, Western Michigan University, USA. | ||||
Abstract | ||||
Many biological studies have indicated that coumarin and its derivatives can act as chemoperventive agents. In this study, three synthetic coumarin derivatives were investigated for their free radical scavenging, anticancer and cell signaling activities. Compound A showed antioxidant effects with 100% decoloration of DPPH at concentration of more than 150 µg/ml with IC50 of 102.12 ± 0.15 µg/ml. Compound B and C induced antioxidant effects with 100% decoloration at 100 and 50 µg/ml, respectively. Also, the IC50 of compounds B and C was 28.35 ± 0.04 and 6.12 ± 0.05 µg/ml, respectively. Moreover, these compounds suppressed PC-3 prostate and MDA-MB-453 breast cancer cells proliferation with IC50 of 20 µM, 70 µM and 50 µM, respectively for PC-3 cells. However, it needs higher dosage to inhibit MDA-MB-453 breast cancer cell growth with IC50 at 25 µM, 137 µM and 100 µM, respectively. All of these compounds activated the apoptotic death signals downstream by activating caspase pathway. And showed many apoptotic characterizations including, DNA fragmentation and apoptotic bodies formation. | ||||
Keywords | ||||
Antioxidant; Anticancer; Free radical scavenging activity; Synthetic coumarin derivatives; Apoptosis; Prostate cancer; Breast cancer | ||||
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