BROCCOLI GROWTH, ANTIOXIDANT ACTIVITY AND HEALTH TISSUES RESPONSES TO ASCORBIC ACID UNDER DEFICIT IRRIGATION | ||||
Fayoum Journal of Agricultural Research and Development | ||||
Article 8, Volume 30, Issue 2, July 2016, Page 106-133 PDF (1.48 MB) | ||||
Document Type: Research articles. | ||||
DOI: 10.21608/fjard.2016.191751 | ||||
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Authors | ||||
Ashraf Sh. Osman1; Mohamed H. Abdel Wahed2; Mofreh S. Tolba1; Kareem K. A. Khalafallah1 | ||||
1Horticulture Department, Faculty of Agriculture, Fayoum University, 63514 Fayoum, Egypt | ||||
2Soils and Water Department (Agric. Eng.), Faculty of Agriculture, Fayoum University, 63514 Fayoum, Egypt | ||||
Abstract | ||||
Two field experiments were conducted during 2013/2014 and 2014/2015 in a private Farm, Ibshway district, Fayoum, Egypt to study deficit irrigation (60, 80 and 100% ETc) and ascorbic acid foliar application (0, 200 and 400 mg l-1) on growth, relative water content (RWC), membrane stability index (MSI), electrolyte leakage (EL), leaf photosynthetic pigments (LPS), osmoprotectants (OS) and DPPH radical-scavenging activity of broccoli plants. The obtained results clarified that, irrigation amount at 80% or 100% ETc, significantly, resulted in higher mean values of stem length and diameter, number of leaves plant-1, leaf MSI, RWC, carotenoids, DPPHradical-scavenging and total free amino acids content than irrigation amount at 60% ETc. On other side, irrigation amounts, irrespective the level used, did not reflect any noticeable impact on number of branches plant-1 and head contents of ascorbic acid. Increasing irrigation water from 60% up to 100% ETc decreased the contents of leaf EL, chlorophyll A, B, A + B, anthocyanin, free proline and TSS. Exogenous application of ascorbic acid at concentrations of 200 and 400 mg l-1, significantly improved most studied growth parameters, RWC, MSI, anthocyanin, DPPH, total free amino acids and endogenous ascorbic acid however, leaf EL decreased gradually. Moreover, the impact of ascorbic acid, irrespective of the concentration used, on leaf photosynthetic pigment, free proline and total soluble sugars contents was at par. | ||||
Keywords | ||||
Deficit irrigation; Ascorbic acid; Antioxidant activity; Broccoli growth; RWC; MIS; EL; leaf photosynthetic pigments; osmoprotectants and DPPH radical-scavenging activity | ||||
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