Preparation and Evaluation of Xylose Palmitate Ester Using Candida antarctica Lipase B as A Reusable Biocatalyst | ||||
| Egyptian Journal of Chemistry | ||||
| Article 7, Volume 62, Issue 3, March 2019, Page 469-474 PDF (490.93 K) | ||||
| Document Type: Original Article | ||||
| DOI: 10.21608/ejchem.2018.5160.1458 | ||||
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| Authors | ||||
Fatma Loulou1; Nacer Rezgui2; Bidjou-Haiour Chahra  3
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| 1LOMPOP. Department of chemistry. | ||||
| 2LOMOP. Department of chemistry. | ||||
| 3Chemistry. UBMA. Bp 12 el hadjar. 23000 annaba | ||||
| Abstract | ||||
| The activity and stability of lipase B from Candida antarctica were investigated in direct enzymatic esterification of D-xylose with palmitic acid using ethylmethylketone as reaction media. The xylose palmitate was obtained under the optimized conditions, 1:1 molar ratio of xylose:palmitic acid, at 250 rpm and 60°C. Effect of the use of Butanol to wash the immobilized lipase before the enzyme reuse was evaluated. The lipase could be successfully reused four times without loss of activity. Various physicochemical characteristics of 1-O-hexadecanoyl-D- xylopyranose were also studied. | ||||
| Keywords | ||||
| Xylose; Lipase; reuse; activity; Surface active properties | ||||
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