Virulence Traits and Antimicrobial Sensitivity Testing of Untyped Mycoplasma species Recovered from Sheep and Goat in Egypt
Osman, M., Abu Elmakarem Mohamed, M., N. Deif, H., M. Osman, K. (2021). Virulence Traits and Antimicrobial Sensitivity Testing of Untyped Mycoplasma species Recovered from Sheep and Goat in Egypt. EKB Journal Management System, 6(4), 39-45. doi: 10.21608/javs.2021.88356.1095
Mona M. Osman; Manal Abu Elmakarem Mohamed; Heba N. Deif; Kamelia M. Osman. "Virulence Traits and Antimicrobial Sensitivity Testing of Untyped Mycoplasma species Recovered from Sheep and Goat in Egypt". EKB Journal Management System, 6, 4, 2021, 39-45. doi: 10.21608/javs.2021.88356.1095
Osman, M., Abu Elmakarem Mohamed, M., N. Deif, H., M. Osman, K. (2021). 'Virulence Traits and Antimicrobial Sensitivity Testing of Untyped Mycoplasma species Recovered from Sheep and Goat in Egypt', EKB Journal Management System, 6(4), pp. 39-45. doi: 10.21608/javs.2021.88356.1095
Osman, M., Abu Elmakarem Mohamed, M., N. Deif, H., M. Osman, K. Virulence Traits and Antimicrobial Sensitivity Testing of Untyped Mycoplasma species Recovered from Sheep and Goat in Egypt. EKB Journal Management System, 2021; 6(4): 39-45. doi: 10.21608/javs.2021.88356.1095

Virulence Traits and Antimicrobial Sensitivity Testing of Untyped Mycoplasma species Recovered from Sheep and Goat in Egypt

Journal of Applied Veterinary Sciences
Article 7, Volume 6, Issue 4, October 2021, Page 39-45  XML PDF (550.2 K)
Document Type: Original Article
DOI: 10.21608/javs.2021.88356.1095
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Authors
Mona M. Osman1; Manal Abu Elmakarem Mohamed2; Heba N. Deif email 3; Kamelia M. Osman4
1Mycoplasma Department, Animal Health Research Institute, ARC., Giza, Egypt.
2Mycoplasma Department, Animal Health Research Institute, ARC, Giza, Egypt
3Department of Microbiology, Faculty of Veterinary Medicine, Cairo University, Egypt.
4Microbiology Department, Faculty of Veterinary Medicine, Cairo University, Giza, Egypt
Abstract
Many Mycoplasma species are involved in caprine and ovine pneumonia all over the world causing serious economic losses. These species have been reported as a cause of pneumonia, mastitis, arthritis, conjunctivitis and genital infection. The detection of Mycoplasma species in the respiratory system of sheep and goat was the objective of the present study. So, a total of 400 samples (250 from sheep and 150 from goat) were examined bacteriologically, biochemically; PCR, and by sequencing; in addition to the antibiotic susceptibility profiles screening. Only 13 isolates could be identified as Mycoplasma species. One of these sequences was submitted to the Genbank taking the accession number MK910041; Mycoplasma species 'ovine/caprine serogroup 11'. Only 7 isolates were weak biofilm producer and the other 6 isolates were non biofilm producers; 8 isolates were positive for catalase test and 5 were negative. H2S production was recorded in 10 isolates; hemolysis was detected in only 6 isolates. In addition, the minimum inhibitory concentrations (MICs) for seven antimicrobial agents, including danofloxacin, tulathromycin, tylosin, streptomycin, lincomycin, florfenicol and oxytetracycline were determined. All the isolates (100%) were sensitive to tulathromycin and tylosin, streptomycin and oxytetracycline, 38.5% of them were sensitive to danofloxacin and 69.2% were sensitive to florfenicol while 69.2% of isolates were resistant to lincomycin. As all the isolates were recovered from apparently healthy as well as clinically diseased animals and identified as untyped Mycoplasma species, So, more investigations will be done to full identify these isolates and discover their roles in infection.
Keywords
Biofilm-formation; Goat; Sheep; Untyped-Mycoplasma-species; Virulence-genes
References
ABDEL HALIUM, M. M., SALIB, F. A., MAROUF, S. A., ABDEL MASSIEH, E. S. 2019. Isolation and molecular characterization of Mycoplasma spp. in sheep and goats in Egypt, Veterinary World. 12(5): 664-670. doi: 10.14202/vetworld.2019.664-670.

ADEHANA, R. K., AJUWAPE, A. T. P., ADETOSOYE, A. I., and ALAKA, O. O. 2006. Characterization of mycoplasmas isolated from pneumonic lungs of sheep and goats. Small Rumin. Res. 63: 44-49.
AZIZI S., TAJBAKHSH, E., REZAII, A., Sh. NEKOUEI, Sh., NAMJOO, A. R. 2011. The role of Mycoplasma ovipneumoniae and Mycoplasma arginini in pneumonic lungs of slaughtered sheep Revue Méd. Vét. 162(6): 310-315. Electronic ISSN      0035-1555.

BESSER, T. E., CASSIRER, E. F., HIGHLAND, M. A., WOLFF, P., JUSTICE-ALLEN, A., MANSFIELD, K., DAVIS, M. A., and FOREYT, W. 2013. Bighorn sheep pneumonia: sorting out the cause of a polymicrobial disease. Prev. Vet. Med. 108: 85e93. DOI: 10.1016/j.prevetmed.2012.11.018

CHINEDU, A. A., and ROGER D. A., and KENNEDY F. Ch. and Stephen I. O. 2014. Studies into the prevalence of Mycoplasma species in small ruminants in Benue State, North-central Nigeria. Trop Anim Health Prod. 46:1087-1092. PMID: 24828562. DOI: 10.1007/s11250-014-0613-6.

COLE, B. C., WARD, J. R., and MARTIN, C. H. 1968. Hemolysin and peroxide activity of Mycoplasma species. J Bacteriol. 95: 2022-2030. PMID: 4876125; PMCID: PMC315129.

DaMASSA, A. J., WAKENELL, P. S., and BROOKS, D. L. 1992. Mycoplasmas of sheep and goat. J. Vet. Diagn. Invest. 4:101-113. DOI: 10.1177/104063879200400126.

EL-ASHRAM, S., HASHAD, M. E., ABDEL-ALIM, G. A., ABDELHAMID, T., and DEIF, H. N. 2021. Seroprevalence of mycoplasmosis in broiler, layer, and native chickens in Giza, Egypt. PLoS One. 12:16(7):e0254220. doi: 10.1371/journal.pone.0254220. PMID: 34252126. https://doi.org/10.1371/JOURNAL.PONE.0254220.

ERNO, H. 1987. Mycoplasmosis of ruminants: a general introduction. Revue Scientifique et Technique, Office International des Epizooties.6: 553-563. DOI: 10.20506/rst.6.3.307.

FELDE, O., KISS, K., BIKSI, I., JERZSELE, Á., and GYURANECZ, M. A. 2018. sertések Mycoplasma hyopneumoniae okozta tüdő gyulladása. (Pneumonia of pigs caused by Mycoplasma hyopneumoniae) Magy Állatorvosok Lapja. 140:337-348. URI: http://hdl.handle.net/10832/2680

FREUNDT, E. A. 1973. Principles of Mycoplasma classification. Ann. N.Y. Acad. Sci. 225: 713. PMID: 7287406.

GRAND, D. L., SARAS, E., BLOND, D., SOLSONA, M. and POUMARAT, F., 2004. Assessment of PCR for routine identification of species of the Mycoplasma mycoides cluster in ruminants. Vet. Res. 35: 635-649. DOI: 10.1051/vetres:2004037.

GROßHENNIG, S., TILL, I. J. G., BUSSE, J., FEUSSNER, I., and STULKE, J. 2016. Hydrogen sulfide is a novel potential virulence factor of Mycoplasma pneumoniae: characterization of the unusual cysteine desulfurase/desulfhydrase HapE Molecular Microbiology. 100(1): 42-54. PMID: 26711628. DOI: 10.1111/mmi.13300.

HIROSE, K., KOBAYASHI, H., ITO, N., KAWASAKI, Y., ZAKO, M., KOTANI, K., OGAWA, H., and SATO, H. 2003. Isolation of mycoplasmas from nasal swabs of calves affected with respiratory diseases and antimicrobial susceptibility of their isolates. Journal of Veterinary Medicine B. 50:347-351. PMID: 14535934 DOI: 10.1046/j.1439-0450.2003.00681.x.

IKHELOA, J. O., AJUWAPE, A. T. P., and ADETOSOYE, A. I. 2004. Biochemical characterization and serological identification of mycoplasmas, isolated from pneumonic lungs of goats slaughtered in abattoirs in Northern Nigeria. Small Rumin. Res. 52:93-97. ISSN: 0921-4488. 

KILIC, A., KALENDER, H., EROKSUZ, H., MUZ, A., and TASDEMIR, B. 2013. Identification by culture, PCR, and immunohistochemistry of mycoplasmas and their molecular typing in sheep and lamb lungs with pneumonia in Eastern Turkey Trop Anim Health Prod. 45:1525-1531. doi: 10.1007/s11250-013-0394-3.

MAKSIMOVIĆ, Z., CHRISTIAN, D. F. E., and MAID, R. 2013. Presence of mycoplasmas in the respiratory system of small ruminants managed under an extensive production system Turk J Vet Anim Sci. 37: 352-354. https://doi.org/10.3906/sag-1209-76.

MCAULIFFE, L., ELLIS, R. J., MILES, K., AYLING, R. D., and NICHOLAS, R. A. 2006. Biofilm formation by Mycoplasma species and its role in environmental persistence and survival. Microbiology.152: 913-922. PMID: 16549656 DOI: 10.1099/mic.0.28604-0

NIANG, M., ROSENBUSCH, R. F., LOPEZ-VIRELLA, J., and KAEBERLE, M. L. 1999. Differential serologic response to Mycoplasma ovipneumoniae and Mycoplasma arginini in lambs affected with chronic respiratory disease. J Vet Diagn Invest. 11: 34-40. PMID: 9925209 DOI: 10.1177/104063879901100105.

NICHOLAS, R., AYLING, R., and MCAULIFFE, L. 2008. Contagious agalactia. In: Mycoplasma Diseases of Ruminants. CABI, Wallingford, UK, 98-113. ePDF 9781845934644.

NICHOLAS, R. A. J. 2017. Mycoplasmology: the big issuesAnim Husb Dairy Vet Sci. 1(1): 1-4. DOI:10.15761/AHDVS.1000102.

OIE, 2017. OIE Terristerial Manual 2017. OIE Manual of Diagnostic Tests and Vaccines for Terrestrial Animals seventh Edition, 1(2): 1404.

PRITCHARD, R. E., and MITCHELL, F. B. 2015. Mycoplasma iowae: relationships among oxygen, virulence, and protection from oxidative stress Veterinary Research. 46:36. DOI: https://doi.org/10.1186/s13567-015-0170-7

SHATALIN, K., SHATALINA, A., MIRONOV, A., and NUDLER, E. 2011. H2S: a universal defense against antibiotics in bacteria. Science. 334: 986-990. PMID: 22096201. DOI: 10.1126/science.1209855

TAMURA, K., STECHER, G., PETERSON, D., FILIPSKI, A., and KUMAR, S. 2013. MEGA6: Molecular Evolutionary Genetics Analysis version 6.0. Molecular Biology andEvolution.30: 2725-2729. doi: 10.1093/molbev/mst197.

TERLAAK, E. A., NOORDERGRAAF, J. H., and VERSCHURE, M. H. 1993. Susceptibilities of Mycoplasma bovis, Mycoplasma dispar, and Ureaplasma diversum strains to antimicrobial agents in vitro.  Antimicrob. Agents Chemother. 37: 317. DOI: 10.1128/AAC.37.2.317. 

VAN KUPPEVELD, F. J., VAN DER LOGT, J. T., ANGULO, A. F., VAN ZOEST, M. J., QUINT, W. G., NIESTERS, H. G., GALAMA, J. M., and MELCHERS, W. J. 1993. Genus- and species-specific identification of mycoplasmas by 16S rRNA amplification. Appl Environ Microbiol. 58(8):2606-15. doi: 10.1128/aem.58.8.2606-2615.1992. Erratum in: Appl Environ Microbiol. 1993 Feb. 59(2):655. PMID: 1381174; PMCID: PMC195828. PMID: 1381174 PMCID: PMC195828 DOI: 10.1128/aem.58.8.2606-2615.1992.

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