Synthesis of Rod/Spherical-Gold Nanoparticles (AuNPs) to Overcome Multidrug-Resistant Bacteremia | ||||
| Alexandria Science Exchange Journal | ||||
| Article 11, Volume 42, Issue 3, September 2021, Page 707-720 PDF (503.24 K) | ||||
| Document Type: Original Article | ||||
| DOI: 10.21608/asejaiqjsae.2021.193090 | ||||
 View on SCiNiTO | ||||
| Authors | ||||
Alia Abed El-Baky Shoeib   1; Awatif A. Hindi* 2; Mashail F.S. Alsayed* 3
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| 1Plant Pathology Department, Faculty of Agriculture, Alexandria University, Alexandria, Egypt. | ||||
| 2Department of Physics and Astronomy, College of Science, King Saud University, Saudi Arabia. | ||||
| 3Department of Botany and Microbiology, College of Science, King Saud University, Saudi Arabia. | ||||
| Abstract | ||||
| Multidrug-resistant bacteremia 15 G-ve and 1 G+ve isolates were collected from inpatients and outpatients departments at Security Forces Hospital in Riyadh, Saudi Arabia. In studying the multidrug-resistance to antibiotics, it was recorded that the G-ve isolates of E. coli (11093623) & (11161644) were resistance to 19 out of 20 tested antibiotics except antibiotic(CN), while the G+ve isolate of MRSA was resistance to 9 out of 18 antibiotics, and it was sensitive to antibiotic (OX and CZ). The Gold Nanoparticles (AuNPs) was examined to discourage the growth of the multidrug-resistance isolates. Rod-AuNPs were the best to discourage the growth of tested bacteria, followed by the Spherical-AuNPs. Swabs were taken from inhibition zone resulting from the inhibitory effect of AuNPs, towards the tested bacteria and re-inoculated on the Nutrient Agar medium, to determine either the bacteriostatic/bactericidal effect for different NPs. The study proved that Rod-AuNPs gave bacteriostatic effect on Pseudomonas aeruginosa (11093627) & (11132667), Enterobacter cloacae (11159053) and Citrobacter freundii(11176991)&(11182565), and gave a bactericidal effect to the rest of the tested isolates. The Spherical-AuNPs gave a bacteriostatic impact of all tested isolates. Electromicroscopic studies disclosed the effect of AuNPs, either rods or spherical-shaped, on the external shape of some of the pathogenic tested bacteria e.g. E. coli, Klebsiella pneumoniae and P. aeruginosaof G-ve bacteria, MRSA of G+ve bacteria. From results obtained, it can be concluded that the nanostructures and morphologies of AuNPs may offer new possibilities for promising therapeutic applications of multidrug-resistance bacteremia. | ||||
| Keywords | ||||
| Rod-Gold Nanoparticles; Spherical-AuNPs; Multidrug-Resistant; Bacteremia; bloodstream; SEM; E. coli; Pseudomonas; Klebsiella; Acinetobacter; Stenotrophomonas; Salmonella; Citrobacter | ||||
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