FEASIBILITY OF A RAPID LATERAL FLOW TEST FOR SIMULTANEOUS DETECTION OF GIARDIA LAMBLIA AND CRYPTOSPORIDIUM PARVUM IN DUODENAL ASPIRATES OF PATIENTS SUFFERING FROM CHRONIC LIVER DISEASES AND ELIGIBLE FOR UPPER ENDOSCOPY | ||||
Journal of the Egyptian Society of Parasitology | ||||
Article 9, Volume 51, Issue 2, August 2021, Page 305-312 PDF (952.15 K) | ||||
Document Type: Original Article | ||||
DOI: 10.21608/jesp.2021.193310 | ||||
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Authors | ||||
MARWA A. ELMALLAWANY1; MOUSA A. MOUSA ISMAIL1; SAMAR S. ATTIA1; RASHA AHMED2; MOHAMED N. ALKADY2; SHAIMAA ELKHOLY3; AHMED SHAKER3; DINA O. HELMY4; REHAM K. NAHNOUSH1 | ||||
1Department of Medical Parasitology | ||||
2Department of Tropical Medicine | ||||
3Department of Internal Medicine | ||||
4Department of Pathology | ||||
Abstract | ||||
Chronic liver diseases (CLDs) represent an important health issue in developing countries and are commonly associated with impaired immunity. This increases the susceptibility to various infectious agents including parasitic infections, which should be properly managed to avoid life threatening complications. This study assessed the feasibility of rapid, easy and applicable screening test for Giardia and Cryptosporidium within 150 CLDs patients suitable for upper endoscopic examination. Stool samples, duodenal aspirates and duodenal biopsies were examined for G. lamblia and C. parvum by different diagnostic techniques. The results showed stool microscopy (13.3% & 7.3%), duodenal aspirate microscopy (5.3% & 4.7%), rapid lateral flow immune- chromatographic assay (RLFIA) applied on duodenal aspirate samples (16.7% & 10%), duodenal biopsies histopathological examination (6.7% & 5.3%) and direct fluorescent antigen detection in stools (16.7% & 9.3%) for giardiasis and cryptosporidiosis respectively. The high sensitivity of lateral flow immune-chromatographic assay in detecting Giardia and Cryptosporidium in duodenal fluid samples proved a good screening test for these patients. | ||||
Keywords | ||||
Egypt; Chronic liver disease patients; Giardia; Cryptosporidium; Immunofluocence; duodenal aspirate; rapid immune-chromatography | ||||
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