Vinpocetine Suppresses Inflammatory and Oxidative Machineries in Acute Model of Inflammation—Pivotal Role of COX-2 Signaling | ||||
| SVU-International Journal of Veterinary Sciences | ||||
| Article 4, Volume 4, Issue 3 - Serial Number 9, September 2021, Page 51-69 PDF (1.25 MB) | ||||
| Document Type: Research article | ||||
| DOI: 10.21608/svu.2021.70729.1119 | ||||
 View on SCiNiTO | ||||
| Authors | ||||
wael Twafik1; Osama Morad2; Reham Hassan3; Khaled nematallah4; Dina El-hamed5; Samy Morad6; Noha Abdelmageed  7
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| 1Department of Biochemistry, Animal Health Research Institute (AHRI), Qena branch, Qena, Egypt | ||||
| 2Neurology and Psychological Medicine department, Faculty of Medicine, Sohag University | ||||
| 3Forensic Medicine and Toxicology Department, Faculty of Veterinary Medicine, South Valley University, Qena, 83523, Egypt | ||||
| 4Department of Pharmacognosy, Faculty of Pharmacy, British University in Egypt (BUE), Cairo, | ||||
| 5Poultry Research Department, Animal Health Research Institute (AHRI), Qena branch, Qena, Egypt | ||||
| 6Department of Pharmacology, Faculty of Veterinary Medicine, South Valley University, Qena, 83523, Egypt | ||||
| 7Department of Pharmacology, Faculty of Veterinary Medicine, Sohag University, Sohag, Egypt | ||||
| Abstract | ||||
| The presence of inflammation is one of the key factors in the onset and progression of various medical disorders and diseases, making it a crucial challenge for treatment. The purpose of this study is to evaluate antioxidant and anti-inflammatory effects of Vinpocetine, using acute models of inflammation; Xylene-, Carrageenan (CGN)-induced inflammation and acetic acid-induced vascular- permeability in mice were used. We also employed molecular docking approach to verify the underlying mechanism of anti-inflammatory activity of Vinpocetine. In a current investigation, Vinpocetine showed anti-inflammatory and antioxidant effects on Xylene- and CGN-induced inflammation in a dose-dependent manner. Vinpocetine reduced inflammatory edema and restored antioxidant tissue balance, probably via suppression of IL-1β, IL-6, TNF-α, MDA and MPO activity, and also through increased non-enzymatic antioxidant (GSH) levels in paw tissue. Histopathological examination showed that Vinpocetine restored normal skin architecture with significant inhibition of tissue edema, congestion, and cellular infiltration in CGN-challenged paw. Mechanistically, Vinpocetine showed downregulation the expression of COX-2 protein, in western blot assessment, that was associated with significant decrease in the level of prostaglandin E 2 (PGE2) levels; a major metabolic product of COX-2 enzyme. Interestingly, Silico study showed that Vinpocetine has strong affinity to COX-2, similar to diclofenac, suggested possible mechanism of downregulation of COX-2 expression. Therefore, Vinpocetine showed antioxidant and anti-inflammatory responses might, in part, due to inhibition of COX-2/PGE 2 pathway. | ||||
| Keywords | ||||
| Vinpocetine; Oxidative stress; COX-2/PGE 2 pathway; Cytokines | ||||
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