The Impact of Programmed Death-1 on Immune Thrombocytopenia
aboalwafa, H., Bardis, D., ahmed, H., Hefney, H., Allam, A. (2021). The Impact of Programmed Death-1 on Immune Thrombocytopenia. EKB Journal Management System, 25(2), 112-120. doi: 10.21608/smj.2021.78198.1256
hasnaa A. aboalwafa; Doaa S.M. Bardis; heba A. ahmed; Hesham M. Hefney; Ahmed A. Allam. "The Impact of Programmed Death-1 on Immune Thrombocytopenia". EKB Journal Management System, 25, 2, 2021, 112-120. doi: 10.21608/smj.2021.78198.1256
aboalwafa, H., Bardis, D., ahmed, H., Hefney, H., Allam, A. (2021). 'The Impact of Programmed Death-1 on Immune Thrombocytopenia', EKB Journal Management System, 25(2), pp. 112-120. doi: 10.21608/smj.2021.78198.1256
aboalwafa, H., Bardis, D., ahmed, H., Hefney, H., Allam, A. The Impact of Programmed Death-1 on Immune Thrombocytopenia. EKB Journal Management System, 2021; 25(2): 112-120. doi: 10.21608/smj.2021.78198.1256

The Impact of Programmed Death-1 on Immune Thrombocytopenia

Sohag Medical Journal
Article 15, Volume 25, Issue 2, April 2021, Page 112-120  XML PDF (857.77 K)
Document Type: Original Article
DOI: 10.21608/smj.2021.78198.1256
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Authors
hasnaa A. aboalwafa1; Doaa S.M. Bardis email 2; heba A. ahmed3; Hesham M. Hefney4; Ahmed A. Allam3
1clinical and chemical pathology department, medical faculty, sohag university , sohag
2clinical and chemical pathology department faculty of medicine sohag university
3clinical and chemical pathology faculty of medicine sohag university
4chemical and clinical pathology , faculty of medicine , Sohag university , Sohag , Sohag
Abstract
Abstract:
Background programmed death (PD-1) has an important role in inhibiting the immune system in immune thrombocytopenic purpura (ITP) by switching off auto-reactive T-cells. This work aims to assess the expression of (PD)-1 negative co-stimulatory molecule in patients with ITP and to detect its relation with platelet count. Patient and methods, forty patients were newly diagnosed as ITP 13 males and 27 females were included, their age ranged from 1 to 43 years and twenty-six healthy subjects 8 males and 18 females as control group their age ranged from 5 to 62 year. Expression of PD-1+ CD4+ T-cells by B.D FACS Calibur flow cytometry was performed in ITP patients and healthy subjects on peripheral blood samples, Mo Abs supplied by BD Bioscience, United States. PD-1 FITC Lot: GR 145543-6 and CD4 PE Cat: 555347, San Jose, California. Negative mouse isotypic control (PE Lot: 0279236, FITC Lot: 0273533). Results, percentages of PD-1 on CD4+T cells in peripheral blood from patients with ITP were considerably higher than that from healthy subjects (p < 0.001), it has no predictive risk for thrombocytopenia Odd's ratio ( 3.773 ) p ( 0.187), CI ( 0. 525 – 27.09). Conclusion: Increased expression of PD-1 molecule on CD-4 T-cells as inhibitory signals to the activated immune system against self-antigens an important part of ITP pathogenesis. Has no predictive role in the degree of thrombocytopenia.
Keywords
ITP; PD-1; CD4; Immune; Inhibition
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