The Collateral Damage of Sodium Nitrates and Alleviating Properties of Vitamin C on Thyroid Gland of Adult Male Albino Rats: Toxicological, Histological and Ultrastructural study | ||||
| Ain Shams Journal of Forensic Medicine and Clinical Toxicology | ||||
| Article 10, Volume 21, Issue 2, July 2013, Page 98-110 PDF (14.22 MB) | ||||
| Document Type: Original Article | ||||
| DOI: 10.21608/ajfm.2013.19376 | ||||
 View on SCiNiTO | ||||
| Authors | ||||
| Zienab Gouda1; Mahmoud Ismail2; Eman Askar3; Zakaria El-Zawahry4 | ||||
| 1Histology and Cell Biology Department,Faculty of Medicine, Zagazig University, Zagazig, Egypt. | ||||
| 2Department of Forensic Medicine and Clinical Toxicology, Faculty of Medicine, Zagazig University, Zagazig, Egypt. | ||||
| 3Histology and Cell Biology Department, Faculty of Medicine, Zagazig University, Zagazig, Egypt | ||||
| 4Department of Forensic Medicine and Clinical Toxicology, Faculty of Medicine, Zagazig University, Zagazig, Egypt | ||||
| Abstract | ||||
| Introduction: In the recent years, a considerable attention has been paid to the problem of nitrates due to their intensive use as agricultural fertilizers which reach to humans and animals by different routes. Purpose: To validate the toxic effects of nitrates in a short-term experiment (60 days) on thyroid gland and the possible ameliorative effect(s) of vitamin C. Materials and methods: Thirty two adult male albino rats were divided into four groups: group I (control) received distilled water, group II received 500mg∕kgvitamin C, group III received 130mg/kg sodium nitrates (NaNO3) and group < strong> IV received 130mg/kg NaNO3 plus 500mg∕kgvitamin C.All supplementations were taken as oral single dose daily for 60 days.At the end of experiment, blood samples were subjected to hormonal assay (T3, T4 and TSH serum levels). Also, thyroid glands' specimens were prepared for the optical and electron microscopic examination. Results: I- The biochemical analyses showed a significant decrease in serum T3 and T4 and a significant increase in TSH. II-Light microscopic examination of nitrates treated group revealed disturbed thyroid architecture in the form of heterogeneous poorly developed follicles. Enlarged deformed follicles with scanty vacuolated colloid, degenerative empty follicles and some fused follicles with exfoliated cells filled their lumen were observed. III-Electron microscopic examination showed stratification of follicular epithelial cells. Most of the follicular cells appeared with irregular nuclei, dilation of rER, many vacuoles and an apparent number of lysosomes. Also hypertrophy and hyperplasia of parafollicular-C cells were observed. An improvement of thyroid gland’s structure, ultrastructure and function was observed in vitamin C added group. In conclusion: This study concluded that nitrate is a thyroid gland disruptor via a mutual interdependence between the follicular, parafollicular and intra-thyroidal mast cells. The supplementation of vitamin C showed beneficial effects upon biochemical and patho-morphological alterations, developed in thyroid gland following exposure to toxic doses of nitrates. So, the recommendations are limitation of sodium nitrates uses, encourage dietary intake of vitamin C and routine analysis of thyroid function in the most nitrates' contaminated areas. | ||||
| Keywords | ||||
| Sodium nitrates; Thyroid gland; vitamin c; Rats; Toxicological; Ultrastructure | ||||
|
Statistics Article View: 195 PDF Download: 313 |
||||
