Effect of Lactobacillus Plantarum on virulence factors of Pseudomonas aeruginosa isolated from wound infection
Shams Eldeen, M., Elnahal, A., Ads, A., Elsawaf, M., Samy, S. (2021). Effect of Lactobacillus Plantarum on virulence factors of Pseudomonas aeruginosa isolated from wound infection. EKB Journal Management System, 2(4), 790-796. doi: 10.21608/mid.2021.92514.1185
Maii Atef Shams Eldeen; Ayat Elnahal; Abdelreheem Ads; Mohamed Elsawaf; Sara Mina Samy. "Effect of Lactobacillus Plantarum on virulence factors of Pseudomonas aeruginosa isolated from wound infection". EKB Journal Management System, 2, 4, 2021, 790-796. doi: 10.21608/mid.2021.92514.1185
Shams Eldeen, M., Elnahal, A., Ads, A., Elsawaf, M., Samy, S. (2021). 'Effect of Lactobacillus Plantarum on virulence factors of Pseudomonas aeruginosa isolated from wound infection', EKB Journal Management System, 2(4), pp. 790-796. doi: 10.21608/mid.2021.92514.1185
Shams Eldeen, M., Elnahal, A., Ads, A., Elsawaf, M., Samy, S. Effect of Lactobacillus Plantarum on virulence factors of Pseudomonas aeruginosa isolated from wound infection. EKB Journal Management System, 2021; 2(4): 790-796. doi: 10.21608/mid.2021.92514.1185

Effect of Lactobacillus Plantarum on virulence factors of Pseudomonas aeruginosa isolated from wound infection

Microbes and Infectious Diseases
Article 22, Volume 2, Issue 4, November 2021, Page 790-796  XML PDF (514.61 K)
Document Type: Original Article
DOI: 10.21608/mid.2021.92514.1185
View on SCiNiTO View on SCiNiTO
Authors
Maii Atef Shams Eldeen email orcid 1; Ayat Elnahal2; Abdelreheem Ads3; Mohamed Elsawaf4; Sara Mina Samy3
1Assistant professor of Medical Microbiology and Immunology, Faculty of Medicine, Tanta University, Egypt.
2Assistant lecturer at Department of Medical Microbiology and Immunology, Faculty of Medicine, Kafr-Elsheik University, Egypt
3Department of Medical Microbiology and Immunology, Faculty of Medicine,Tanta University, Egypt
4Department of General Surgery, Faculty of Medicine, Tanta University,Egypt
Abstract
Background: Pseudomonas aeruginosarepresents a major concern in nosocomial infections.  It possesses manyvirulence factors that aid its pathogenicity and antimicrobial resistance. Non-antibiotic therapy against this pathogen includes probiotics, phages, phytomedicine and other new modalities. These agents alone or in combination with antibiotics can be highly effective against these virulent strains. Objectives: To investigate the in-vitro effect exerted by the probiotic “Lactobacillus plantarum” on two virulence factors of Pseudomonas aeruginosa (pyocyanin pigment production and elastase enzyme activity). Methods: The study was carried out on 40 Pseudomonas aeruginosa strains isolated from pus collected from wound infection using sterile swabs. The effect of Lactobacillus plantarum on pyocyanin production and the elastolytic activity of Pseudomonas aeruginosa were detected using pyocyanin extraction method and Human Elastase ELISA Kit respectively before and after the addition of Lactobacillus plantarum. Results: Among the forty strains investigated, the pyocyanin production and elastolytic activity of Pseudomonas aeruginosa isolates were significantly decreased after addition of Lactobacillus plantarum. Conclusions: Lactobacillus plantarum has an important invitro anti-pathogenic effect against Pseudomonas aeruginosa as it significantly interferes with two of its main virulence factors (pyocyanin & elastase).
Keywords
Pseudomonas aeruginosa; Virulence; Probiotic; Lactobacillus plantarum
Statistics
Article View: 549
PDF Download: 377