Isolation and Molecular Identification of Avian Pathogenic Escherichia coli in Broiler Chickens Suffering from Colibacillosis in some Governorates in Egypt
Abu El Hammed, W., Soufy, H., EL-Shemy, A., Nasr, S., Dessouky, M. (2022). Isolation and Molecular Identification of Avian Pathogenic Escherichia coli in Broiler Chickens Suffering from Colibacillosis in some Governorates in Egypt. EKB Journal Management System, 53(1), 31-41. doi: 10.21608/ejvs.2021.93246.1274
Waleed Abu El Hammed; Hamdy Soufy; Ahmed EL-Shemy; Soad Nasr; Mohamed I. Dessouky. "Isolation and Molecular Identification of Avian Pathogenic Escherichia coli in Broiler Chickens Suffering from Colibacillosis in some Governorates in Egypt". EKB Journal Management System, 53, 1, 2022, 31-41. doi: 10.21608/ejvs.2021.93246.1274
Abu El Hammed, W., Soufy, H., EL-Shemy, A., Nasr, S., Dessouky, M. (2022). 'Isolation and Molecular Identification of Avian Pathogenic Escherichia coli in Broiler Chickens Suffering from Colibacillosis in some Governorates in Egypt', EKB Journal Management System, 53(1), pp. 31-41. doi: 10.21608/ejvs.2021.93246.1274
Abu El Hammed, W., Soufy, H., EL-Shemy, A., Nasr, S., Dessouky, M. Isolation and Molecular Identification of Avian Pathogenic Escherichia coli in Broiler Chickens Suffering from Colibacillosis in some Governorates in Egypt. EKB Journal Management System, 2022; 53(1): 31-41. doi: 10.21608/ejvs.2021.93246.1274

Isolation and Molecular Identification of Avian Pathogenic Escherichia coli in Broiler Chickens Suffering from Colibacillosis in some Governorates in Egypt

Egyptian Journal of Veterinary Sciences
Article 5, Volume 53, Issue 1, April 2022, Page 31-41  XML PDF (1.28 MB)
Document Type: Original Article
DOI: 10.21608/ejvs.2021.93246.1274
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Authors
Waleed Abu El Hammed email orcid 1; Hamdy Soufyorcid 2; Ahmed EL-Shemyorcid 1; Soad Nasrorcid 2; Mohamed I. Dessoukyorcid 3
1Department of Parasitology and Animal Diseases, National Research Centre, 33 Bohouth Street, Dokki, Giza, 12622, Egypt.
2Department of Parasitology and Animal Diseases, National Research Centre, 33 Bohouth Street, Dokki, Post Box 12622, Giza, Egypt.
3Department of Clinical Pathology, Faculty of Veterinary Medicine – Cairo University
Abstract
This study aimed to evaluate the most predominant strain of avian pathogenic Escherichia coli (APEC) that causes colibacillosis with respiratory manifestation in broiler chickens. Isolation and serotyping identification for the isolates of E. coli were performed. Prevalence of APEC differentiated by detection of the 8 virulence-associated genes “astA, iss, irp2, papC iucD, tsh, vat, and cva/cvi” using multiplex PCR technique in the most important isolates of E. coli was also investigated. Antibacterial sensitivity test for antibiotics disc and oregano at different concentrations (20%, 15%, 10%, and 5%), were estimated for the most important isolates of E. coli. A total of 50 broiler farms were located in Gharbia, Giza, Menoufia, Bahaira, and Dakahliah Governorates were examined. Specimens from lung, liver, spleen, and kidney from diseased birds were used for bacteriological examination. Results revealed that E. coli O27 was the most predominant serotype strain (11 out of 35 isolates), followed by the serotypes of O146 (4/35), O Untypable serotypes (4/35), and the (1/35) of each serotype of O8, O20, O44, O78, O114, O125, O142, O152, O153, O158, O164, and O166. One isolate of APEC O27 out of 11 isolates has 6 virulence-related genes “astA, iss, papC, iucD, vat, and cva/cvi”. Difloxacin and oregano at a concentration of 20% have the highest percentage (100%) of sensitivity against 11 isolates of E. coli O27 strains. In conclusion, E. coli O27 was the most predominant serotype infecting farms of broiler chickens. Difloxacin and oregano 20% could be used for the treatment of APEC O27.

Keywords
Molecular; Avian pathogenic E. coli O27; Antibacterial sensitivity test; Broiler chickens
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