Modulation of resolution of CCl4 induced liver cirrhosis following gadolinium chloride induced Kupffer cell blockade in an experimental model in male mice
El-Hadidy, M., Abbas, A., Elmenabawy, F., Abd-Elaziz, G. (2016). Modulation of resolution of CCl4 induced liver cirrhosis following gadolinium chloride induced Kupffer cell blockade in an experimental model in male mice. EKB Journal Management System, 6(1), 97-105. doi: 10.21608/sjdfs.2016.194545
Mona Gaber El-Hadidy; Amr Medhat Abbas; Fayza Elmenabawy; Gad El Mawla Abd-Elaziz. "Modulation of resolution of CCl4 induced liver cirrhosis following gadolinium chloride induced Kupffer cell blockade in an experimental model in male mice". EKB Journal Management System, 6, 1, 2016, 97-105. doi: 10.21608/sjdfs.2016.194545
El-Hadidy, M., Abbas, A., Elmenabawy, F., Abd-Elaziz, G. (2016). 'Modulation of resolution of CCl4 induced liver cirrhosis following gadolinium chloride induced Kupffer cell blockade in an experimental model in male mice', EKB Journal Management System, 6(1), pp. 97-105. doi: 10.21608/sjdfs.2016.194545
El-Hadidy, M., Abbas, A., Elmenabawy, F., Abd-Elaziz, G. Modulation of resolution of CCl4 induced liver cirrhosis following gadolinium chloride induced Kupffer cell blockade in an experimental model in male mice. EKB Journal Management System, 2016; 6(1): 97-105. doi: 10.21608/sjdfs.2016.194545

Modulation of resolution of CCl4 induced liver cirrhosis following gadolinium chloride induced Kupffer cell blockade in an experimental model in male mice

Scientific Journal for Damietta Faculty of Science
Volume 6, Issue 1, June 2016, Page 97-105  XML PDF (1.02 MB)
Document Type: Original articles
DOI: 10.21608/sjdfs.2016.194545
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Authors
Mona Gaber El-Hadidy email 1; Amr Medhat Abbas2; Fayza Elmenabawy3; Gad El Mawla Abd-Elaziz3
1Assiatant lectuer of Medical Physiology, Faculty of Medicine, Mansoura University
2Assiatant Professor of Medical Physiology, Faculty of Medicine, Mansoura University
3Professor of Medical Physiology, Faculty of Medicine, Mansoura University.
Abstract
Liver cirrhosis is a major cause of morbidity and mortality worldwide. There is evidence indicating that liver cirrhosis is dynamic and can be bidirectional, involving phases of progression and regression, along with major changes in the regulation of matrix degradation. There is also evidence that Kupffer cells participate in both fibrogenesis and fibrolysis. This study was aiming to determine the effect of inhibition of Kuppfer cells by gadolinium chloride (GdCl3) on CCl4-induced liver cirrhosis in male balb-c mice. GdCl3 injected i/p at dose of 10 mg /kg dissolved in normal saline, three times per week for 2weeks, after injection of mice with CCl4 i/p at dose of 0.4 ml /kg (v/v) with olive oil twice per week for 6 weeks. The rise in serum levels of alanine aminotransferase (ALT), aspartate aminotransferase (AST), and alkaline phosphatase (ALP) in CCl4-intoxicated mice was markedly suppressed by GdCl3 and histopathological changes were reduced and the expression of transforming growth factor β (TGF-β1) and Kupffer cells marker (CD68) were reduced in GdCl3treated mice. The results of this study indicate that activated Kupffer cells are involved in both process of fibrogensis as they could release cytotoxic mediators outside the cell that cause hepatocyte damage and fibrinolysis through release of gelatinases enzyme.
Keywords
Liver cirrhosis; olive oil; cytotoxic mediators; gelatinases enzyme
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