C-Banding Karyotype and Molecular Characterization on Cumin, Caraway and Coriander | ||||
| Annals of Agricultural Science, Moshtohor | ||||
| Article 63, Volume 59, Issue 5, 2021, Page 513-514 PDF (163.6 K) | ||||
| Document Type: Original Article | ||||
| DOI: 10.21608/assjm.2021.195300 | ||||
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| Abstract | ||||
| Our goal for this study to characterize three species germplasm of family Apiaceae, namely (cumin, caraway and coriander), to make a chromosome characterization and molecular fingerprint for the mentioned species. Results performed on Karyomorphological showed that; Cuminum cyminum L. (cumin: 2n=14), Carum carvi (caraway: 2n=20) and Coriandrum sativum (coriander: 2n=22). The total Chromatin Relative Length percentage (RL%) showed cumin ± 10.69 μm ± 19.70 μm, caraway ± 7.30 μm ± 13.40 μm and coriander ± 5.58 μm ± 12.07 μm. Satellites in all the cases were associated to short arms. The molecular characterization for the three species (caraway, cumin, and coriander) was conducted using 5 AFLP combinations and 15 anchored-ISSR primers. The total amplified bands were 330 (162 ISSR+168 AFLP), with an average 83.75% (89.5 ISSR+78 AFLP) per primer. The combined dendrogram based on both AFLP and SSR markers for the three accessions was divided into 2 main clusters; the first cluster has 2 accessions (caraway and cumin) with 60% similarity, while coriander falls in a distinct cluster. | ||||
| Keywords | ||||
| Cytogenetic; Cytology; C-banding; Chromosome; Cumin; Caraway; Coriander; AFLP; ISSR molecular marker | ||||
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