Effect of gonadotrophin dose on oocytes yield and quality and embryonic development in Super ovulated mice
Zaahkouk, S., Abdon, A., Al-Atrash, A., Kandil, O., Ammar, A., Yones, A. (2018). Effect of gonadotrophin dose on oocytes yield and quality and embryonic development in Super ovulated mice. EKB Journal Management System, 73(9), 7557-7565. doi: 10.21608/ejhm.2018.19577
Samir A. M. Zaahkouk; Ahmed S. Abdon; Ahmed M. Al-Atrash; Omaima M. Kandil; Amal A. A. Ammar; Asmaa A. M. Yones. "Effect of gonadotrophin dose on oocytes yield and quality and embryonic development in Super ovulated mice". EKB Journal Management System, 73, 9, 2018, 7557-7565. doi: 10.21608/ejhm.2018.19577
Zaahkouk, S., Abdon, A., Al-Atrash, A., Kandil, O., Ammar, A., Yones, A. (2018). 'Effect of gonadotrophin dose on oocytes yield and quality and embryonic development in Super ovulated mice', EKB Journal Management System, 73(9), pp. 7557-7565. doi: 10.21608/ejhm.2018.19577
Zaahkouk, S., Abdon, A., Al-Atrash, A., Kandil, O., Ammar, A., Yones, A. Effect of gonadotrophin dose on oocytes yield and quality and embryonic development in Super ovulated mice. EKB Journal Management System, 2018; 73(9): 7557-7565. doi: 10.21608/ejhm.2018.19577

Effect of gonadotrophin dose on oocytes yield and quality and embryonic development in Super ovulated mice

The Egyptian Journal of Hospital Medicine
Article 22, Volume 73, Issue 9, October 2018, Page 7557-7565  XML PDF (657.59 K)
Document Type: Original Article
DOI: 10.21608/ejhm.2018.19577
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Authors
Samir A. M. Zaahkouk1; Ahmed S. Abdon2; Ahmed M. Al-Atrash3; Omaima M. Kandil2; Amal A. A. Ammar4; Asmaa A. M. Yones4
1Faculty of science, Al-Azhar University
2Reproduction and Artificial Insemination, Veterinary Research Division, National Research Center
3Medical Administration, Nuclear Materials Authority
4Medical and Radiation Research Dept., Nuclear Materials Authority, Cairo, Egypt
Abstract
Background: Hormonal control for multiple follicular growths in IVF program was directed toward maximizing the yield of fertilizable oocytes, enhance successful implantation and improve early embryonic development.
Aim: The effect of different doses of urinary human menopausal gonadotropin (U-hMG) in superovulation regimen was studied.
Materials and Methods: Four groups of immature female mouse were sequentially injected by 5 IU, 10 IU, 15 IU and 20 IU of U-hMG followed by injection of 15 IU human chorionic gonadotropin (hCG) for all groups. Mature cumulus oocytes complexes (COCs) were collected 12 hours later then the grading of cumulus cell expansion and oocytes maturity was determined. In vitro fertilization for mature oocytes was done by using epididymal sperm, the embryonic development was followed up till the blastocyst stage. Results: Results demonstrated that the super ovulatory response in immature mice increased with increasing dose of hMG. 15 IU U-hMG was the optimal dose that gave maximum number oocytes with higher maturation rate,minimum degeneration rate and support embryonic development.
Conclusion: 15 IU is the optimal dose for induction of superovulation and support in vitro embryo developmentin immature female mice.
Keywords
Immature mice; HMG dose; Maturation rate; Cleavage rate; Embryo development
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