Box-Behnken Design Assisted Development and Optimization of RP-HPLC-PDA Technique for Determination of Cannabidiol in the Bulk and Nanoformulation
Ahmed, B., Rizwanullah, M., Usmani, Z., Mir, S., Amin, S. (2022). Box-Behnken Design Assisted Development and Optimization of RP-HPLC-PDA Technique for Determination of Cannabidiol in the Bulk and Nanoformulation. EKB Journal Management System, 65(3), 295-304. doi: 10.21608/ejchem.2021.82528.4072
Bakr Ahmed; Md. Rizwanullah; Zakiya Usmani; Showkat Mir; Saima Amin. "Box-Behnken Design Assisted Development and Optimization of RP-HPLC-PDA Technique for Determination of Cannabidiol in the Bulk and Nanoformulation". EKB Journal Management System, 65, 3, 2022, 295-304. doi: 10.21608/ejchem.2021.82528.4072
Ahmed, B., Rizwanullah, M., Usmani, Z., Mir, S., Amin, S. (2022). 'Box-Behnken Design Assisted Development and Optimization of RP-HPLC-PDA Technique for Determination of Cannabidiol in the Bulk and Nanoformulation', EKB Journal Management System, 65(3), pp. 295-304. doi: 10.21608/ejchem.2021.82528.4072
Ahmed, B., Rizwanullah, M., Usmani, Z., Mir, S., Amin, S. Box-Behnken Design Assisted Development and Optimization of RP-HPLC-PDA Technique for Determination of Cannabidiol in the Bulk and Nanoformulation. EKB Journal Management System, 2022; 65(3): 295-304. doi: 10.21608/ejchem.2021.82528.4072

Box-Behnken Design Assisted Development and Optimization of RP-HPLC-PDA Technique for Determination of Cannabidiol in the Bulk and Nanoformulation

Egyptian Journal of Chemistry
Article 27, Volume 65, Issue 3, March 2022, Page 295-304  XML PDF (954.45 K)
Document Type: Original Article
DOI: 10.21608/ejchem.2021.82528.4072
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Authors
Bakr Ahmed1; Md. Rizwanullah1; Zakiya Usmani2; Showkat Mirorcid 2; Saima Amin email orcid 3
1Formulation Research Laboratory, Department of Pharmaceutics, School of Pharmaceutical Education and Research, Jamia Hamdard, New Delhi–110062, India.
2Phytopharmceutical Laboratory, Department of Pharmacognosy and Phytochemistry, School of Pharmaceutical Education and Research, Jamia Hamdard, New Delhi–110062, India.
3Dept. of Pharmaceutics, School of Pharmaceutical Education and Research, Jamia Hamdard, New Delhi 110062
Abstract
The focus of this research was to implement a new RP-HPLC-PDA method for determining cannabidiol (CBD), which has been established with the help of Quality by Design (QbD). Design expert (version 12) software was used to conduct the factor screening studies. The mobile phase ratio, flow rate, and temperature conditions were used as independent variables, while retention time, peak area, and peak tailing were used as dependent variables in a Box-Behnken design. The best separation was obtained with acetonitrile and water as mobile phase in the ratio of 45:55 % (v/v), a flow rate of 1.0 ml/min, and an oven temperature of 30 °C with PDA detection at 210 nm. The newly optimized method showed the concentration linearity range in µg/ml (1187.57 - 5997.83) of CBD with an excellent correlation coefficient R2 = 0.9951. The % recovery of the CBD was obtained between 97.84 to 99.34 %, and the % RSD was not more than 2 %, thus clearly confirming the high level of accuracy for analysis. The technique was assessed under the ICH guidelines, which revealed excellent linearity, precision, and robustness. Consequently, the approach was used to determine the retention time of the drug in its nanoemulsion formulation, which revealed no substantial change in retention time.
Keywords
Cannabidiol; RP-HPLC; ICH guidelines; Box–Behnken design; Quality by Design
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