Screening for Group B Streptococci in Pregnant women by culture and polymerase chain reaction methods
Ghandour, A., Youssef, A., Mohamad, W., Bakry, R., Mammdoh, Y., Fathy, R. (2021). Screening for Group B Streptococci in Pregnant women by culture and polymerase chain reaction methods. EKB Journal Management System, 30(4), 47-54. doi: 10.21608/ejmm.2021.197454
Aliaa M.A. Ghandour; Ahmed A. Youssef; Wegdan A. Mohamad; Rania M. Bakry; Yousra M Mammdoh; Rawhia Fathy. "Screening for Group B Streptococci in Pregnant women by culture and polymerase chain reaction methods". EKB Journal Management System, 30, 4, 2021, 47-54. doi: 10.21608/ejmm.2021.197454
Ghandour, A., Youssef, A., Mohamad, W., Bakry, R., Mammdoh, Y., Fathy, R. (2021). 'Screening for Group B Streptococci in Pregnant women by culture and polymerase chain reaction methods', EKB Journal Management System, 30(4), pp. 47-54. doi: 10.21608/ejmm.2021.197454
Ghandour, A., Youssef, A., Mohamad, W., Bakry, R., Mammdoh, Y., Fathy, R. Screening for Group B Streptococci in Pregnant women by culture and polymerase chain reaction methods. EKB Journal Management System, 2021; 30(4): 47-54. doi: 10.21608/ejmm.2021.197454

Screening for Group B Streptococci in Pregnant women by culture and polymerase chain reaction methods

Egyptian Journal of Medical Microbiology
Article 7, Volume 30, Issue 4, October 2021, Page 47-54  XML PDF (432.26 K)
Document Type: New and original researches in the field of Microbiology.
DOI: 10.21608/ejmm.2021.197454
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Authors
Aliaa M.A. Ghandour email 1; Ahmed A. Youssef2; Wegdan A. Mohamad1; Rania M. Bakry3; Yousra M Mammdoh4; Rawhia Fathy1
1Medical Microbiology and Immunology Department, Faculty of Medicine, Assiut University, Assiut, Egypt
2Department of Obstetrics and Gynecology, Faculty of Medicine, Assiut University, Assiut, Egypt
3Department of Oncological Clinical Pathology,South Egypt Cancer Institute, Assiut University, Assiut, Egypt
4Department of Clinical Pathology, Faculty of Medicine, Assiut University, Assiut, Egypt
Abstract
Background: Group B Streptococcus (GBS) inhabits pregnant women's genitourinary tracts and spreads to the offspring. Objective: This study aimed to screen for GBS colonization in pregnant women, to compare between GBS culture, VITEK® 2 and PCR, and to assess the antimicrobial susceptibility of GBS isolates. Methodology: Vaginal and rectal swabs were collected from 82 pregnant women. GBS was isolated by culture on Strep B Select. Confirmation by VITEK® 2 and PCR for atr and 16S rRNA genes was done. Results: Sixteen vaginal (19.5%) and 22 rectal colonized cases (26.8%) were positive for GBS by culture on Strep B Select agar. GBS was detected in 10 vaginal (12.2%) and 16 rectal colonized cases (19.5%) by VITEK® 2 and PCR for both atr and 16S rRNA genes.  Conclusion: It is suggested that pregnant women should be screened for GBS colonization.  The best precise tests to detect GBS are PCR and VITEK® 2.
Keywords
GBS; VITEK® 2; PCR; atr and 16S rRNA genes
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