Matrix-Assisted Laser Desorption Ionization-Time of Flight Mass Spectrometry (MALDI-TOF MS) for the Identification of Bacteria Causing Urinary Tract Infections
Mahmoud, A., Fathy, L., Fahim, N., El-Sadek, W. (2021). Matrix-Assisted Laser Desorption Ionization-Time of Flight Mass Spectrometry (MALDI-TOF MS) for the Identification of Bacteria Causing Urinary Tract Infections. EKB Journal Management System, 30(4), 103-112. doi: 10.21608/ejmm.2021.197468
Ahmed G. T. Mahmoud; Lamia F. Fathy; Nehal M.A. Fahim; Walaa Abd El-Latif El-Sadek. "Matrix-Assisted Laser Desorption Ionization-Time of Flight Mass Spectrometry (MALDI-TOF MS) for the Identification of Bacteria Causing Urinary Tract Infections". EKB Journal Management System, 30, 4, 2021, 103-112. doi: 10.21608/ejmm.2021.197468
Mahmoud, A., Fathy, L., Fahim, N., El-Sadek, W. (2021). 'Matrix-Assisted Laser Desorption Ionization-Time of Flight Mass Spectrometry (MALDI-TOF MS) for the Identification of Bacteria Causing Urinary Tract Infections', EKB Journal Management System, 30(4), pp. 103-112. doi: 10.21608/ejmm.2021.197468
Mahmoud, A., Fathy, L., Fahim, N., El-Sadek, W. Matrix-Assisted Laser Desorption Ionization-Time of Flight Mass Spectrometry (MALDI-TOF MS) for the Identification of Bacteria Causing Urinary Tract Infections. EKB Journal Management System, 2021; 30(4): 103-112. doi: 10.21608/ejmm.2021.197468

Matrix-Assisted Laser Desorption Ionization-Time of Flight Mass Spectrometry (MALDI-TOF MS) for the Identification of Bacteria Causing Urinary Tract Infections

Egyptian Journal of Medical Microbiology
Article 13, Volume 30, Issue 4, October 2021, Page 103-112  XML PDF (750.99 K)
Document Type: New and original researches in the field of Microbiology.
DOI: 10.21608/ejmm.2021.197468
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Authors
Ahmed G. T. Mahmoud1; Lamia F. Fathy2; Nehal M.A. Fahim2; Walaa Abd El-Latif El-Sadek email 3
1Specialist of Medical Microbiology, Central lab of armed force, Egypt
2Professor of Medical Microbiology and Immunology Department, Ain Shams University, Egypt
3Medical Microbiology &Immunology ,Ain Shams University ,Cairo ,Egypt
Abstract
Background: Matrix assisted laser desorption/ionization-time of flight (MALDI-TOF) technology is a technique which allows microbial identification in clinical microbiology laboratories based on microbial protein profiles. A promising application of the MALDI-TOF technique comprises carrying out identification from direct urine samples. Objectives: The aim of this study is to evaluate the performance of MALDI-TOF MS system for the rapid identification of clinically relevant urinary tract pathogens in urine samples to the species level. Methodology: two hundred (200) urine samples with findings suggestive of urinary tract infections (UTI) were identified directly by MALDI-TOF MS, and same samples were identified from culture on MALDI-TOF MS. 73 colonies from 73 samples were further identified by Bd Phoneix and PCR. Results: MALDI-TOF MS directly from samples identified 174 samples (91 to the species level 83 to genus level), while MALDI-TOF MS after culture identified 191 samples (187 to the level of species, and 4 to genus level). In addition, 9 samples gave no results from the direct method and no growth in culture. The sensitivity and specificity of direct method compared to after culture method was 91.1% and 100%, respectively. Colonies of 73 samples were detected by the BD Phoneix automated identification system and by PCR. The results were identical among the three systems. Conclusion: The ability of MALDI-TOF MS’s to detect microorganisms from urine samples directly can provide early diagnosis of UTIs and avoid the inadequate or unnecessary empirical antimicrobial treatment. 
Keywords
MALDI-TOF MS; Urinary Tract infection; BD Phoenix
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