The in Vitro Effect of Vitamin D on the Antibiotic Susceptibility of Carbapenem Resistant Pseudomonas species
sahloul, N., Kamel, N., Shoeib, S., Gohar, N. (2021). The in Vitro Effect of Vitamin D on the Antibiotic Susceptibility of Carbapenem Resistant Pseudomonas species. EKB Journal Management System, 30(4), 121-127. doi: 10.21608/ejmm.2021.197470
Nahla Yassin sahloul; Nehad Kamel; Samira Shoeib; Noha M. Gohar. "The in Vitro Effect of Vitamin D on the Antibiotic Susceptibility of Carbapenem Resistant Pseudomonas species". EKB Journal Management System, 30, 4, 2021, 121-127. doi: 10.21608/ejmm.2021.197470
sahloul, N., Kamel, N., Shoeib, S., Gohar, N. (2021). 'The in Vitro Effect of Vitamin D on the Antibiotic Susceptibility of Carbapenem Resistant Pseudomonas species', EKB Journal Management System, 30(4), pp. 121-127. doi: 10.21608/ejmm.2021.197470
sahloul, N., Kamel, N., Shoeib, S., Gohar, N. The in Vitro Effect of Vitamin D on the Antibiotic Susceptibility of Carbapenem Resistant Pseudomonas species. EKB Journal Management System, 2021; 30(4): 121-127. doi: 10.21608/ejmm.2021.197470

The in Vitro Effect of Vitamin D on the Antibiotic Susceptibility of Carbapenem Resistant Pseudomonas species

Egyptian Journal of Medical Microbiology
Article 15, Volume 30, Issue 4, October 2021, Page 121-127  XML PDF (519.21 K)
Document Type: New and original researches in the field of Microbiology.
DOI: 10.21608/ejmm.2021.197470
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Authors
Nahla Yassin sahloul email 1; Nehad Kamel2; Samira Shoeib2; Noha M. Gohar2
1Medical Microbiology and Immunology, Cairo University
2Department of Medical Microbiology and Immunology, Faculty of Medicine, Cairo University, Cairo, Egypt
Abstract
Background: Carbapenem-resistant Pseudomonas infections become a serious problem with limited options of treatment. Many studies support the role of vitamin D in competing different infections by many mechanisms through enhancing host immune defense. Objective: to assess the antimicrobial effect of vitamin D on carbapenem-resistant Pseudomonas. Methodology: Fifty carbapenem-resistant Pseudomonas isolates were studied. All isolates were tested for susceptibility to imipenem and meropenem using disk diffusion method. Modified carbapenem inactivation methods were done for carbapenemase and metallo-beta-lactamase detection. Isolates were tested for MIC of meropenem and vitamin D by broth-microdilution and their combination by checkerboard method. Results: Regarding the combination between meropenem and vitamin D; 10 isolates showed decrease in their MIC of meropenem. One isolate showed additive result, 5 isolates showed decrease more than 2 folds, 4 isolates showed 2 folds decrease. Conclusion: Vitamin D acts as an adjuvant to meropenem rather than having a direct antimicrobial effect on carbapenem-resistant Pseudomonas species.
Keywords
Pseudomonas; carbapenem resistance; meropenem; vitamin D; checkerboard
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