Evaluation of Antimicrobial Potential of Tetradecane Extracted from Pediococcus acidilactici DSM: 20284 - CM Isolated from Curd Milk. | ||||
Egyptian Journal of Chemistry | ||||
Article 67, Volume 65, Issue 3, March 2022, Page 705-713 PDF (478.04 K) | ||||
Document Type: Original Article | ||||
DOI: 10.21608/ejchem.2021.92658.4385 | ||||
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Authors | ||||
zeinab said nasr 1; hanan El-shershaby2; Khaled Mohamed Sallam3; Nermine Abed4; Ibrahim Abd- El ghany2; Nagwa sidkey4 | ||||
1egyption atomic energy authority | ||||
2egyptian atomic energy authority | ||||
3Kafer al mandara Aga DKHLYA, AlOboor city | ||||
4faculty of science, Al- azhar university | ||||
Abstract | ||||
This study was oriented to production of bioactive tetradecane reported in extracts of Pediococcus acidilactici isolated from curd milk and 16S rRNA sequence analysis of isolate showed 98% correlation with P. acidilactici DSM: 20284-CM (accession no. NR042057.1). P. acidilactici activity has been tested by disc diffusion assay against Staphylococcus aureus MRSA ATCC 43300, Listeria monocytogenes ATCC 19116, Pseudomonas aeruginosa MTCC 1934, and Escherichia coli MTCC 1610, Clostridium bifermentans MTCC 11273 and Candida albicans MTCC 183. The highest antibacterial and antifungal activities created by this strain were obtained. Tetradecane active metabolite which was extracted by ethyl acetate contained one observed bioactive spot on thin layer chromatography plate. Spectroscopic analyses confirm the presence of tetradecane as pure product. Minimal inhibitory concentrations (MIC) of tetradecane against indicators are noteworthy. In conclusion, this is the first work show tetradecane was obtained from a novel P. acidilactici-CM strain. Tetradecane act as antibacterial and antifungal. | ||||
Keywords | ||||
curd milk; Pediococcus acidilactici; ethyl acetate extraction; tetradecane; antimicrobial activity | ||||
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