PROPAGATION OF SOME HARD–TO–ROOT ORNAMENTAL PLANTS BY TISSUE CULTURE II – ESTABLISHMENT AND MULTIPLICATION | ||||
Fayoum Journal of Agricultural Research and Development | ||||
Article 10, Volume 19, Issue 1, January 2005, Page 91-107 PDF (487.89 K) | ||||
Document Type: Research articles. | ||||
DOI: 10.21608/fjard.2005.197791 | ||||
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Authors | ||||
Mahmoud El Sayed Hashem1; Faisal Mohamed Saadawy2; Salah AbdelAziz Gomaa2; Nermeen El–Sayed Abdul-Moneem2 | ||||
1Dept. of Horticulture, Faculty of Agriculture, Ain Shams University, Shoubra ElKhema, Cairo, Egypt. | ||||
2Dept. of Ornamental Plant Researches, Horticulture Research Institute, Agriculture Research Center, Giza, Egypt. | ||||
Abstract | ||||
Applying benzyladenine (BA) at 1 or 2 ppm to the culture medium at the establishment stage, proved to have a significant effect in increasing survival percentage of Cupressus macrocarpa explants. On the other hand, survival percentage of Conocarpus explants was not significantly influenced by the presence or absence of BA. The use of BA resulted also in the highest multiplication rates of both Cupressus macrocarpa and Conocarpus erectus, though the effect was insignificant with the first species. Leaving a heel on the explant of Conocarpus erectus showed significantly a superior effect in increasing shoot number, shoot fresh weight and leaf number. At the multiplication stage, the highest number of shoots resulted by using BA at 2 and 4 ppm for Cupressus macrocarpa and Conocarpus erectus, respectively. The highest contents of total chlorophyll and carotenoids of Cupressus macrocarpa shoots were those induced by applying kinetin at 6 ppm. Application of BA at 6 ppm to the culture medium was associated with a greater increase in these contents in the case of Conocarpus erectus. | ||||
Keywords | ||||
Cupressus macrocarpa; Conocarpus erectus; Tissue culture; Benzyladenine; Kinetin; Establishment; Multiplication | ||||
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