Effects of vitamin C on structural changes in the tissues of the giant African catfish, Heterobranchus longifilis exposed to Zinc Oxide nanoparticles
D. Owolabi, O., I. Abdulkareem, S. (2021). Effects of vitamin C on structural changes in the tissues of the giant African catfish, Heterobranchus longifilis exposed to Zinc Oxide nanoparticles. EKB Journal Management System, 25(5), 103-129. doi: 10.21608/ejabf.2021.197872
Olufemi D. Owolabi; Saratu I. Abdulkareem. "Effects of vitamin C on structural changes in the tissues of the giant African catfish, Heterobranchus longifilis exposed to Zinc Oxide nanoparticles". EKB Journal Management System, 25, 5, 2021, 103-129. doi: 10.21608/ejabf.2021.197872
D. Owolabi, O., I. Abdulkareem, S. (2021). 'Effects of vitamin C on structural changes in the tissues of the giant African catfish, Heterobranchus longifilis exposed to Zinc Oxide nanoparticles', EKB Journal Management System, 25(5), pp. 103-129. doi: 10.21608/ejabf.2021.197872
D. Owolabi, O., I. Abdulkareem, S. Effects of vitamin C on structural changes in the tissues of the giant African catfish, Heterobranchus longifilis exposed to Zinc Oxide nanoparticles. EKB Journal Management System, 2021; 25(5): 103-129. doi: 10.21608/ejabf.2021.197872

Effects of vitamin C on structural changes in the tissues of the giant African catfish, Heterobranchus longifilis exposed to Zinc Oxide nanoparticles

Egyptian Journal of Aquatic Biology and Fisheries
Article 7, Volume 25, Issue 5, September and October 2021, Page 103-129  XML PDF (943.09 K)
Document Type: Original Article
DOI: 10.21608/ejabf.2021.197872
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Authors
Olufemi D. Owolabi; Saratu I. Abdulkareem
Abstract
Heterobranchus longifilis juveniles (n = 450, Mean length ± SD = 10.28 ± 1.34 cm) were exposed to varying concentrations (0 = control, 6.00, 8.00, 10.00, 12.00 mg/l) of zinc oxide nanoparticles (ZnO-NPs) for 60 days to determine its effects on the histopathology of the gill and liver. To evaluate recovery from ZnO-NPs exposure, fish were subjected to a 30-day depuration. Ascorbic acid (AA) ameliorative potential against ZnO-NPs toxicity was assessed by augmenting fish basal diet with different AA (50.00, 250.00, 500.00, 1000.00 mg.kg-1) levels. After each experiment, fish gills and liver were removed for histopathological analysis. Gills of exposed fish revealed different alterations like epithelial hyperplasia, epithelial lifting, lamellar fusion, oedema, aneurysms, and necrosis; while alterations such as nuclear vacuolation, fatty degeneration, cytoplasmic degeneration, focal fibrosis and necrosis were recorded in the liver. The mean degree tissue change (DTC) in both organs of ZnO-NPs-exposed fish gradually increased with an increase in ZnO-NPs concentration and exposure period. After 30-day depuration, some of the lesions in both organs attained recovery, while others did not. AA administration to ZnO-exposed fish at 500 mg.kg-1 completely reversed the lesions in both organs compared to the normal tissue architecture in the control. The results suggest that ZnO-NPs could trigger structural alterations in both the branchial and hepatic tissues of H. longifilis, and full recovery under depuration was slow. However, 500 mg.kg-1 AA dietary supplementation have the propensity to ameliorate the ZnO-NPs-induced pathological lesions in H. longifilis.
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