THE ROLE OF EXOPOLYSACCHARIDE PRODUCED BY Pleurotus Sapidus AS MEDICAL AGENT | ||||
| Delta Journal of Science | ||||
| Article 11, Volume 43, Issue 1, May 2021, Page 133-148 PDF (1.3 MB) | ||||
| Document Type: Research and Reference | ||||
| DOI: 10.21608/djs.2021.197873 | ||||
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| Authors | ||||
| Eman H. F. Abd El-Zaher* 1; Alaa M. Abou Zied1; Youssef M. Mohamed2; Faten M. A. Semana1 | ||||
| 1Botany and Microbiology Department, Faculty of Science, Tanta University, Tanta, Egypt., | ||||
| 2Botany and Microbiology Department, Faculty of Science, Damanhour University, Damanhour, Egypt. | ||||
| Abstract | ||||
| Mushrooms have been used since centuries for the treatment of different diseases and efforts were made to isolate different compounds from the mushrooms responsible for medicinal activities. Among the mushroom genus Pleurotus is worldwide in distribution due to their medicinal properties such interventions ameliorate hepatic lipid profiles, reduce inflammation and oxidative stress, noticeable antioxidant, antiinflammatory, antimicrobial, antitumor and analgesic properties. These properties are attributed mainly to polysaccharides synthesized by Pleurotus. The aim of current study is to improve of Exopolysaccharide production by Pleurotus sapidus through manipulation of different culture and environmental conditions using response surface methodology (RSM). Plackett–Burman design indicated that; shaking, starch, and incubation temperature are the most effective parameters. Box–Behnken RSM indicated that; the optimum concentration for each parameter was: 148.9 rpm for shaking, 30.1g/l starch, and 25.2 ºC for incubation temperature. After production, EPS was extracted, purified, identified using Fourier transform infrared (FTIR), Nuclear Magnetic Resonance (¹HNMR) and High Performance Liquid Chromatography (HPLC). The result indicated that; it consisting of β-(1→3) glucans, β-(1→6) glucans branched, Chitin, Mannans, and Galactans with different concentrations in EPS. Exopolysaccharides extracted from Pleurotus sapidus showed antitumor, antiviral, antibacterial, anticoagulant and antioxidant activity. | ||||
| Keywords | ||||
| Exopolysaccharides; production; Box Benken; factorial design; biological activity; Pleurotus sapidus | ||||
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