Green Synthesis of Zinc oxide Nanocomposite Using Fusarium oxysporum and Evaluation of the Anticancer Effect on Hepatocellular Carcinoma

Rashwan, Doaa Sayed; abd el hamed, may mohamed; El-deen, Mohamed Diaa; afify, mei mohamed; mohamed, mona hassan; mohamed, abd el razik farrag; Nagy, Rana Assem; A. Elhakim, Heba Kamal

doi:10.21608/ejchem.2021.91841.4361

	Green Synthesis of Zinc oxide Nanocomposite Using Fusarium oxysporum and Evaluation of the Anticancer Effect on Hepatocellular Carcinoma
Egyptian Journal of Chemistry
Article 20, Volume 65, Issue 4, April 2022, Page 197-207 PDF (1.17 MB)
Document Type: Original Article
DOI: 10.21608/ejchem.2021.91841.4361
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Authors
Doaa Sayed Rashwan ¹; may mohamed abd el hamed²; Mohamed Diaa El-deen³; mei mohamed afify³; mona hassan mohamed⁴; abd el razik farrag mohamed⁵; Rana Assem Nagy ⁶; Heba Kamal A. Elhakim⁷
¹1 Biochemistry Division, Faculty of Science, Cairo University, Giza, 12613, Egypt 2 Faculty of Medicine, Galala University, Suez, 43511, Egypt
²Spectroscopy Department, National Research Centre, Dokki, Giza, 12622, Egypt
³Department of Biochemistry, Genetic Engineering and Biotechnology Research Division, National Research Centre, Dokki, Giza, 12622, Egypt
⁴Department of Chemistry, Faculty of Science, Cairo University, Giza, 12613, Egypt
⁵Professor of Histology and Histochemistry, Pathology Department, Medical Research Division, National Research Centre, Dokki, Giza, 12622, Egypt
⁶Biochemistry Division, Faculty of Science, Cairo University, Giza, 12613, Egypt
⁷Biochemistry, faculty of Science, Cairo university, Giza, Egypt
Abstract
Nanoparticles can be synthesized by chemical, physical and biological system methods. In this work, Superparamagnetic Iron Oxide@Silver nanoparticles (SPION@Ag NPs) were modified by zinc oxide nanoparticles (ZnO NPs). SPION@Ag core shell NPs were synthesized by the biological method using Fusarium oxysporum fungus and coated with ZnO NPs. This method is eco-friendly and low cost. Characterization techniques such as UV, FTIR, HR-TEM, HR-SEM, XRD and zeta potential were used. The most common type of liver cancer is hepatocellular carcinoma (HCC). Hepatocellular carcinoma was induced by a single dose of diethyl nitrosamine (DEN) 60 mg/Kg b.wt. and was followed after two days with carbon tetra chloride (CCl4) diluted with paraffin oil (50% v/v, 2 ml/Kg b.wt.) twice a week for one month. A histopathology examination was done after scarifying. Clear changes were shown in the comparison between the HCC group treated with ZnO@SPION@Ag nanocomposite and the positive control group. Liver function tests showed a highly significant decrease after using the nanocomposite for HCC treatment. Our study aim is to evaluate the therapeutic anticancer effect of ZnO@SPION@Ag nanocomposite on hepatocellular carcinoma in male albino rats, which was synthesized by a new method called green chemistry or green synthesis.
Keywords
Hepatocellular carcinoma; Drug delivery; Zinc oxide; Nanocomposite; Anticancer


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