Impact of engineered nano silver on plant parasitic nematode and measurement of DNA damage
R.M., S., Abdel-Razik, A., Ibrahim, M., Al-Kordy, M., taha, E. (2022). Impact of engineered nano silver on plant parasitic nematode and measurement of DNA damage. EKB Journal Management System, 65(4), 43-51. doi: 10.21608/ejchem.2021.99035.4605
Shoaib R.M.; A.B. Abdel-Razik; M.M. Ibrahim; M.A. Al-Kordy; Entsar H taha. "Impact of engineered nano silver on plant parasitic nematode and measurement of DNA damage". EKB Journal Management System, 65, 4, 2022, 43-51. doi: 10.21608/ejchem.2021.99035.4605
R.M., S., Abdel-Razik, A., Ibrahim, M., Al-Kordy, M., taha, E. (2022). 'Impact of engineered nano silver on plant parasitic nematode and measurement of DNA damage', EKB Journal Management System, 65(4), pp. 43-51. doi: 10.21608/ejchem.2021.99035.4605
R.M., S., Abdel-Razik, A., Ibrahim, M., Al-Kordy, M., taha, E. Impact of engineered nano silver on plant parasitic nematode and measurement of DNA damage. EKB Journal Management System, 2022; 65(4): 43-51. doi: 10.21608/ejchem.2021.99035.4605

Impact of engineered nano silver on plant parasitic nematode and measurement of DNA damage

Egyptian Journal of Chemistry
Article 6, Volume 65, Issue 4, April 2022, Page 43-51  XML PDF (685.31 K)
Document Type: Original Article
DOI: 10.21608/ejchem.2021.99035.4605
View on SCiNiTO View on SCiNiTO
Authors
Shoaib R.M.orcid 1; A.B. Abdel-Razik email orcid 2; M.M. Ibrahim1; M.A. Al-Kordy1; Entsar H tahaorcid 3
1Genetic and Cytology Dept., Genetic Engineering and Biotechnology Division, National Research Centre, Dokki, Cairo, Egypt.
2Department of Genetics, Faculty of Agriculture, Ain Shams University
3Department of Plant Protection, Faculty of Agriculture, Ain Shams University
Abstract
Plant parasitic nematodes: Meloidogyne incognita, Pratylenchus penetrans, and Tylenchulus semipenetrans are the most genera which cause economic damage in the agricultural production. Nanotechnology is a promising alternative method in nematode control, especially the green synthesis methods. Present study deals with the effects of Ag engineered nanoparticles (AgENPs) synthesized by using plant extract to evaluate juvenile mortality, egg hatching, morphological change, and DNA damage. Five concentrations of AgENPs were prepared from each AgENPs and were added to300 individuals of nematode separately up to five days. Data approved that, Meloidogyne incognita was more affected than Pratylenchus penetrans and Tylenchulus semipenetrans in all the treatments. Curcuma comosa (N1) was the most effective preparation against nematode larvae, followed by Chitosan (N3), Crotalaria juncea (N4), and Cycas circinalis (N2). These effects were increased proportionally with the nano concentration and the exposure time. While N3 recorded the highest reduction in egg hatchability. Some visual changes were observed on the treated larvae and eggs. Likewise, the most DNA damage for nematode larvae was detected with N1,followed by N4, N3, and N2. As a conclusion, AgENPs may provide a better alternative to chemically synthesized against deferent plant parasitic nematode.
Keywords
AgENPs; root-knot nematodes; juvenile mortality; egg hatching; morphological change; DNA damage
Statistics
Article View: 698
PDF Download: 459