The Neuro-protective Role of Vitamin B12 in Diazinon Poisoned Male Wistar Rats: Histopathological and Biochemical Evaluation
Hameed, A., Ahmed, J. (2021). The Neuro-protective Role of Vitamin B12 in Diazinon Poisoned Male Wistar Rats: Histopathological and Biochemical Evaluation. EKB Journal Management System, 52(Issue The 9th International Conference of Veterinary Research Division National Research Centre, Giza, Egypt 27th -29th September 2021), 35-40. doi: 10.21608/ejvs.2021.90194.1264
Aseel Kamil Hameed; Jihad abdulameer Ahmed. "The Neuro-protective Role of Vitamin B12 in Diazinon Poisoned Male Wistar Rats: Histopathological and Biochemical Evaluation". EKB Journal Management System, 52, Issue The 9th International Conference of Veterinary Research Division National Research Centre, Giza, Egypt 27th -29th September 2021, 2021, 35-40. doi: 10.21608/ejvs.2021.90194.1264
Hameed, A., Ahmed, J. (2021). 'The Neuro-protective Role of Vitamin B12 in Diazinon Poisoned Male Wistar Rats: Histopathological and Biochemical Evaluation', EKB Journal Management System, 52(Issue The 9th International Conference of Veterinary Research Division National Research Centre, Giza, Egypt 27th -29th September 2021), pp. 35-40. doi: 10.21608/ejvs.2021.90194.1264
Hameed, A., Ahmed, J. The Neuro-protective Role of Vitamin B12 in Diazinon Poisoned Male Wistar Rats: Histopathological and Biochemical Evaluation. EKB Journal Management System, 2021; 52(Issue The 9th International Conference of Veterinary Research Division National Research Centre, Giza, Egypt 27th -29th September 2021): 35-40. doi: 10.21608/ejvs.2021.90194.1264

The Neuro-protective Role of Vitamin B12 in Diazinon Poisoned Male Wistar Rats: Histopathological and Biochemical Evaluation

Egyptian Journal of Veterinary Sciences
Article 5, Volume 52, Issue The 9th International Conference of Veterinary Research Division National Research Centre, Giza, Egypt 27th -29th September 2021, October 2021, Page 35-40  XML PDF (1.39 MB)
Document Type: Conference
DOI: 10.21608/ejvs.2021.90194.1264
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Authors
Aseel Kamil Hameed email orcid 1; Jihad abdulameer Ahmedorcid 2
1Basic science Dept., College of Dentistry, Uni. of Basrah, Iraq
2Dept. of Pathology, college of veterinary medicine, uni. of Basra, Iraq
Abstract
The protective role of Vit.B12 on neurotoxic effect of diazinon has been studied and conducted on 24 adults male wistar rats were divided equally into 4 groups, the group I was used as normal control; group II was orally given 1/10 LD50 (3.8mg/kg.bw) of diazinon for one month; group III was orally given 1/10 LD50 (3.8mg/kg.bw) of diazinon plus systemic injection (I.M route) of Vit.B12 (4 mg/kg.bw) for one month; group IV was given systemic injection (I.M route) of Vit.B12 (4 mg/kg.bw) only for one month. Biochemically it revealed a significant (P≤0.05) increase in GSH, CAT and SOD values in both Vit.B12 groups (group III and group IV) as well to control group when compared to diazinon group (group II); while the values of MDA and peroxynitrite revealed a significant (P≤0.05) decreases in the both Vit.B12 groups (group III and group IV) as well to control group when compared to diazinon group (group II); moreover, the histopathological results of the diazinon group (group II) revealed a serious neurologic changes included necrotized neurons, diffuse neuronal edema, perivascular edema and hyperplasia of glial cells; while the Vit.B12 groups showed no effective histopathological changes were more or less to control group. There are a little information has been provided concerning the relation between diazinon and Vit.B12,therefore, the current study concluded that the Vit.B12 has a significant protective role against the neurological toxic effects of diazinon on basis of histopathological and biochemical patterns.
Keywords
Histology; Neuron; Vitamin B12; Diazinon; Toxicity
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