ACTIVITY OF HYDROLYSIS ENZYMES IN DIFFERENT INSTAR LARVAE OF THE AMERICAN BOLLWORM, HELICOVERPA ARMIGERA (HUBNER)
, M. S, A., K. W, M., A. A, K., Hend, A, A. (2017). ACTIVITY OF HYDROLYSIS ENZYMES IN DIFFERENT INSTAR LARVAE OF THE AMERICAN BOLLWORM, HELICOVERPA ARMIGERA (HUBNER). EKB Journal Management System, 40(1), 165-179. doi: 10.21608/jes.2017.19893
Abdel-Wahed , M. S; Mahmoud, K. W; Khidr, A. A; Alashry, Hend, A. "ACTIVITY OF HYDROLYSIS ENZYMES IN DIFFERENT INSTAR LARVAE OF THE AMERICAN BOLLWORM, HELICOVERPA ARMIGERA (HUBNER)". EKB Journal Management System, 40, 1, 2017, 165-179. doi: 10.21608/jes.2017.19893
, M. S, A., K. W, M., A. A, K., Hend, A, A. (2017). 'ACTIVITY OF HYDROLYSIS ENZYMES IN DIFFERENT INSTAR LARVAE OF THE AMERICAN BOLLWORM, HELICOVERPA ARMIGERA (HUBNER)', EKB Journal Management System, 40(1), pp. 165-179. doi: 10.21608/jes.2017.19893
, M. S, A., K. W, M., A. A, K., Hend, A, A. ACTIVITY OF HYDROLYSIS ENZYMES IN DIFFERENT INSTAR LARVAE OF THE AMERICAN BOLLWORM, HELICOVERPA ARMIGERA (HUBNER). EKB Journal Management System, 2017; 40(1): 165-179. doi: 10.21608/jes.2017.19893

ACTIVITY OF HYDROLYSIS ENZYMES IN DIFFERENT INSTAR LARVAE OF THE AMERICAN BOLLWORM, HELICOVERPA ARMIGERA (HUBNER)

Journal of Environmental Science
Article 8, Volume 40, Issue 1, December 2017, Page 165-179  XML PDF (301.04 K)
Document Type: Review Article
DOI: 10.21608/jes.2017.19893
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Authors
Abdel-Wahed , M. S1; Mahmoud, K. W1; Khidr, A. A2; Alashry, Hend, A3
1Plant Protection Department, Pesticides Department, Faculty of Agric., Ain Shams University
2Plant Protection Research Institute, Agricultural Research Center, Dokki-Giza, Cairo
3Plant Protection Research Institute, Agricultural Research Center, Dokki-Giza, Cairo.
Abstract
Enzymes activity included acetylcholinesterase, non-specific α and β- esterases and alkaline phosphatase were determined calorimetrically in the whole homogenates of the different instar larvae; 3rd, 4th, 5th and 6th of the field strain as well as the baseline, laboratory strain of American bollworm, Helicoverpa armigera. The results indicated that the enzymes activity levels in the different instar larvae of the field strain were markedly higher than that in the laboratory strain at the respective instar larvae. The enzymatic activity levels increased steady from the 3rd to the 6th instar larvae. A sharp increase in the enzymatic activity levels were determined in the 6th instar larvae. The average levels of the enzymatic activity in both field colony and laboratory strain were 11.630 and 3.627 µg acetyl bromide released / min. /individual for A.Ch.E.; 8.819 and 3.430 µg α-naphthol released/ min/ individual and the corresponding values were ranged between 5.232 and 3.218 µg β– naphthol released / min/ larva for beta esterase; respectively and157.669 and 50.428 unit X 103/larva for alkaline phosphatase; respectively
Keywords
Helicoverpa armigera; field and laboratory strain; enzymes activity
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