MARKER-ASSISTED IDENTIFICATION OF STEM RUST RESISTANCE GENES SR2, SR13, SR22 AND SR24 IN EGYPTIAN WHEAT CULTIVARS | ||||
| Egyptian Journal of Plant Breeding | ||||
| Article 13, Volume 24, Issue 1, March 2020, Page 225-245 PDF (981.38 K) | ||||
| Document Type: Original research | ||||
| DOI: 10.12816/ejpb.2020.199317 | ||||
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| Abstract | ||||
| Wheat stem rust caused by Puccinia graminis Pers. f. sp. tritici Eriks. and E. Henn. (Pgt), is one of the most destructive wheat diseases. It can cause up to 90 % yield loss in wheat production but has been effectively under control due to the successful deployment of resistant genes in wheat cultivars since the 1950s. The identification of molecular markers of flanking disease resistance genes simplifies the identification of stem rust resistance genes. The objective of this work was to identify the stem rust resistance gens Sr2, Sr13, Sr22 and Sr24 in some Egyptian wheat cultivars. Four SSR markers Xgwm533, Xwmc580, Xcfa2123 and Xbarc71 linked to stem rust resistance genes Sr2, Sr13, Sr22 and Sr24, respectively were used to identify these four genes in 38 Egyptian wheat cultivars. The analysis of 38 Egyptian wheat cultivars for markers linked to stem rust resistance genes indicates that Sr2 was present in 32 cultivars, while Sr13 was detected in 18 cultivars, Sr22 was also detected in 7 cultivars and Sr24 wasn’t detected in any cultivar. These markers should be useful in marker-assisted pyramiding of stem rust resistance genes to develop new cultivars with multiple genes resistance against stem rust races in Egypt. | ||||
| Keywords | ||||
| Stem rust; Wheat; SSR markers; Resistance genes | ||||
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