Phenotypic and Molecular Characterization of Yersinia ruckeri and Psychrobacter Species Isolated from Farmed Fishes
El-Sharaby, S., El-Ballal, S., Ali, M., El-Hady, M. (2021). Phenotypic and Molecular Characterization of Yersinia ruckeri and Psychrobacter Species Isolated from Farmed Fishes. EKB Journal Management System, 3(2), 1-7. doi: 10.21608/jcvr.2021.199408
Salma Mohamed El-Sharaby; Salah El-Ballal; Mohamed Ali; Maha El-Hady. "Phenotypic and Molecular Characterization of Yersinia ruckeri and Psychrobacter Species Isolated from Farmed Fishes". EKB Journal Management System, 3, 2, 2021, 1-7. doi: 10.21608/jcvr.2021.199408
El-Sharaby, S., El-Ballal, S., Ali, M., El-Hady, M. (2021). 'Phenotypic and Molecular Characterization of Yersinia ruckeri and Psychrobacter Species Isolated from Farmed Fishes', EKB Journal Management System, 3(2), pp. 1-7. doi: 10.21608/jcvr.2021.199408
El-Sharaby, S., El-Ballal, S., Ali, M., El-Hady, M. Phenotypic and Molecular Characterization of Yersinia ruckeri and Psychrobacter Species Isolated from Farmed Fishes. EKB Journal Management System, 2021; 3(2): 1-7. doi: 10.21608/jcvr.2021.199408

Phenotypic and Molecular Characterization of Yersinia ruckeri and Psychrobacter Species Isolated from Farmed Fishes

Journal of Current Veterinary Research
Article 1, Volume 3, Issue 2, October 2021, Page 1-7  XML PDF (303.09 K)
Document Type: Original Article
DOI: 10.21608/jcvr.2021.199408
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Authors
Salma Mohamed El-Sharaby email orcid 1; Salah El-Ballal2; Mohamed Ali3; Maha El-Hady3
1University of Sadat City Faculty of Veterinary Medicine Dept. of Aquatic Animal Medicine and Management
2Department of Veterinary Pathology, Faculty of Veterinary Medicine, University of Sadat City, Sadat City 32511, Egypt.
3Department of Fish Diseases, Animal Health Research institute, Dokki, ARC, Giza, 12619, Egypt.
Abstract
One of the major threats to aquaculture sector is bacterial diseases. In the current study, samples from different species of cultured fishes were collected from different fish farms at different localities in Menofiya and Kafr El-Sheikh Provinces, Egypt for the detection of causative agents of some disease. Clinically, the infected fishes were inspected for symptoms like darkness of skin and, hemorrhages in different parts of the fish’s body including the base of fins, tail, and rot of fins. Identification of the retrieved bacterial pathogens using conventional biochemical and molecular methods were demonstrated. Based on the phenotypic and biochemical characterization using API20E, API 20 NE and VITEK® 2 compact, the retrieved isolates were identified as Yersinia ruckeri and Psychrobacter species. Molecular characterization was accomplished through polymerase chain reaction (PCR) and confirmed by DNA-sequencing and phylogenetic analysis. The high molecular identity and close phylogenetic relationship confirmed that the isolates were genetically homologous to Yersinia ruckeri and Psychrobacter spp.

Keywords
Yersinia ruckeri; Psychrobacter spp; PCR; Sequencing; phylogenetic analysis
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