Prevalence and PCR Screening of Pseudomonas Isolated from Some Meat Products in Egypt
Elbayoumi, Z., Zahran, R., Shawish, R. (2021). Prevalence and PCR Screening of Pseudomonas Isolated from Some Meat Products in Egypt. EKB Journal Management System, 3(2), 130-136. doi: 10.21608/jcvr.2021.199443
Zakaria Elbayoumi; Rasha Zahran; Reyad Shawish. "Prevalence and PCR Screening of Pseudomonas Isolated from Some Meat Products in Egypt". EKB Journal Management System, 3, 2, 2021, 130-136. doi: 10.21608/jcvr.2021.199443
Elbayoumi, Z., Zahran, R., Shawish, R. (2021). 'Prevalence and PCR Screening of Pseudomonas Isolated from Some Meat Products in Egypt', EKB Journal Management System, 3(2), pp. 130-136. doi: 10.21608/jcvr.2021.199443
Elbayoumi, Z., Zahran, R., Shawish, R. Prevalence and PCR Screening of Pseudomonas Isolated from Some Meat Products in Egypt. EKB Journal Management System, 2021; 3(2): 130-136. doi: 10.21608/jcvr.2021.199443

Prevalence and PCR Screening of Pseudomonas Isolated from Some Meat Products in Egypt

Journal of Current Veterinary Research
Article 14, Volume 3, Issue 2, October 2021, Page 130-136  XML PDF (307.92 K)
Document Type: Original Article
DOI: 10.21608/jcvr.2021.199443
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Authors
Zakaria Elbayoumi email orcid 1; Rasha Zahran2; Reyad Shawishorcid 3
1department of food hygiene faculty of veterinary medicine university of Sadat city
2Department of Bacteriology, Mycology and Immunology, Faculty of Veterinary Medicine, University of Sadat City, Egypt.
3Department of Food Hygiene and Control, Faculty of Veterinary Medicine, University of Sadat City, Egypt.
Abstract
A total of 200 random samples of meat products represented by minced meat, beef burger, beef kofta and beef sausage (50 of each) were collected from different supermarkets at El Menofiya and Cairo Governorates. The collected samples were examined for isolation of Psuedomonas and molecular characterization by using Multiplex PCR. psuedomonas was isolated from examined samples with the percentage of 18% (9) ,30% (15), 26% (13) and 22% (11) in minced meat, beef burger, beef kofta and beef sausage, respectively. The isolated pseudomonas could be serologically identified as P. aeruginosa ,P. diminuta ,P. fluorescens , P. proteolytica, P. fragi , P. putrefaciens and P. putida. Multiplex PCR results showed that the prevalence of virulence genes of P. aeruginosa were exotoxin A gene (ETA) 36%(4), gryase B gene (gyrB) with outer membrane protein gene (oprL) 54% (6), exotoxin A gene (ETA) with outer membrane protein gene (oprL) 18% (2) and gryase B gene (gryB) with exotoxin A gene (ETA) with outer membrane protein gene (oprL) (18%(2). The prevalence of Pseudomonas species in meat products samples is highly significant Therefore, its presence should be avoided during first stages of food processing.

Keywords
Meat products; Psuedomonas; virulence genes
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