Uses of Modified Buffy Coat Method As a Tool for Diagnosis of Bovine Hemoparasites
Al-Badrani, B., hammed, S. (2022). Uses of Modified Buffy Coat Method As a Tool for Diagnosis of Bovine Hemoparasites. EKB Journal Management System, 53(1), 65-73. doi: 10.21608/ejvs.2021.96527.1298
Basima Abdulfatah Al-Badrani; Sara Mohammed hammed. "Uses of Modified Buffy Coat Method As a Tool for Diagnosis of Bovine Hemoparasites". EKB Journal Management System, 53, 1, 2022, 65-73. doi: 10.21608/ejvs.2021.96527.1298
Al-Badrani, B., hammed, S. (2022). 'Uses of Modified Buffy Coat Method As a Tool for Diagnosis of Bovine Hemoparasites', EKB Journal Management System, 53(1), pp. 65-73. doi: 10.21608/ejvs.2021.96527.1298
Al-Badrani, B., hammed, S. Uses of Modified Buffy Coat Method As a Tool for Diagnosis of Bovine Hemoparasites. EKB Journal Management System, 2022; 53(1): 65-73. doi: 10.21608/ejvs.2021.96527.1298

Uses of Modified Buffy Coat Method As a Tool for Diagnosis of Bovine Hemoparasites

Egyptian Journal of Veterinary Sciences
Article 9, Volume 53, Issue 1, April 2022, Page 65-73  XML PDF (1.66 MB)
Document Type: Original Article
DOI: 10.21608/ejvs.2021.96527.1298
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Authors
Basima Abdulfatah Al-Badrani email orcid 1; Sara Mohammed hammed2
1Veterinary internal and preventive Medicine College of Veterinary Medicine University of Mosul , Iraq
2Department of Internal and Preventive Medicine, College of Veterinary Medicine, University of Mosul, Mosul-Iraq.
Abstract
Modified buffy coat method (BCM) using microhematocrit tubes and acridine orange (AO) staining permitsquick(~10 min/sample) and accurate diagnosis of commonbovine hemoparasites compared to the light microscopy(LM) of Giemsa stained smears.Babesia, Theileria,Anaplasma, Ehrlichia and Trypanosoma parasites were detected. It was easy to see Babesia merozoites, trypomastigotes and epimastigotes of Trypanosoma parasite. Similarly, fluorescent microscopy (FLM), offers a method for detecting low parasitemiathat are undetectable by light microscopy. Under LM, the agreement between the twodiagnostic techniquesusing Cohen’s Kappa index was perfect for Trypanosoma (1.000), substantial for Anaplasma(0.615), moderate for Theileria, and Ehrlichia (0.459, 0.433 respectively). BCM was able to detect all positive cases (100%) of Babesia infections using LM and FLM. Modified buffy coat method usingdark field microscopy (DFM) is recommended as a fast and dependable tool to detect Babesiaand Trypanosomaparasites. BCMusing AO stain can be set up directlywitha low cost using anordinary LM.BCM is a valuable tool for diagnosinghemoparasitesmixed infection. Buffy coatmethodcan be used as an alternative technique for the dry stained smear for quickly screening of bovine blood samples.
Keywords
Hemoparasites; buffy coat; Acridine orange; Cows
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